NMR Chemical Shift Assignments of N terminal La motif domain of La protein
MSEETSTQIL KQVEYYFSDS NFPRDKFLRS EAAKNVDNYI SIDVIASFNR MKTISTDLQL ITEALKKSTR LQVSEDGKMV RRLDPLPENI D
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.9 % (903 of 1089) | 74.6 % (426 of 571) | 92.2 % (388 of 421) | 91.8 % (89 of 97) |
Backbone | 95.0 % (513 of 540) | 94.4 % (170 of 180) | 96.0 % (261 of 272) | 93.2 % (82 of 88) |
Sidechain | 74.8 % (478 of 639) | 65.5 % (256 of 391) | 90.0 % (215 of 239) | 77.8 % (7 of 9) |
Aromatic | 21.9 % (14 of 64) | 0.0 % (0 of 32) | 43.8 % (14 of 32) | |
Methyl | 93.0 % (93 of 100) | 86.0 % (43 of 50) | 100.0 % (50 of 50) |
1. La motif
MSEETSTQIL KQVEYYFSDS NFPRDKFLRS EAAKNVDNYI SIDVIASFNR MKTISTDLQL ITEALKKSTR LQVSEDGKMV RRLDPLPENI DSolvent system 90% H2O/10% D2O, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | La-type RNA-binding domain | [U-98% 15N] | 0.37 mM | |
2 | buffer salts | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | La-type RNA-binding domain | [U-98% 13C; U-98% 15N] | 0.37 mM | |
4 | buffer salts | natural abundance | 50 mM |