1H, 13C, and 15N Chemical Shift Assignments for Calcium free, Myristoylated Visinin-like protein 3
MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 76.6 % (1775 of 2317) | 80.5 % (977 of 1214) | 68.8 % (619 of 900) | 88.2 % (179 of 203) |
Backbone | 91.4 % (1046 of 1144) | 93.3 % (364 of 390) | 89.3 % (507 of 568) | 94.1 % (175 of 186) |
Sidechain | 66.1 % (896 of 1355) | 74.3 % (612 of 824) | 54.5 % (280 of 514) | 23.5 % (4 of 17) |
Aromatic | 43.8 % (98 of 224) | 85.7 % (96 of 112) | 0.0 % (0 of 110) | 100.0 % (2 of 2) |
Methyl | 95.3 % (162 of 170) | 95.3 % (81 of 85) | 95.3 % (81 of 85) |
1. VILIP-3 monomer
MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQFSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Myristoylated VILIP-3 | [U-100% 15N] | 0.5 ~ 1.0 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | Myristoylated VILIP-3 | [U-100% 13C; U-100% 15N] | 0.5-1 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Myristoylated VILIP-3 | [U-100% 13C; U-100% 15N] | 0.5-1 mM | |
8 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Myristoylated VILIP-3 | natural abundance | 0.5-1 mM | |
10 | D2O | natural abundance | 100 % |