Solution structure of H-RasT35S mutant protein in complex with Kobe2601
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPSIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQH
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 13.7 % (237 of 1736) | 13.3 % (132 of 992) | 14.1 % (105 of 744) |
Backbone | 6.8 % (56 of 827) | 5.9 % (20 of 340) | 7.4 % (36 of 487) |
Sidechain | 18.9 % (201 of 1064) | 17.2 % (112 of 652) | 21.6 % (89 of 412) |
Aromatic | 3.0 % (4 of 134) | 6.0 % (4 of 67) | 0.0 % (0 of 67) |
Methyl | 75.8 % (144 of 190) | 76.8 % (73 of 95) | 74.7 % (71 of 95) |
1. entity 1
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPSIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHSolvent system 80% D2O/20% DMSO_d6, Pressure 1 atm, Temperature 278 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | H-RasT35S | [U-98% 13C; U-98% 15N] | 1 mM | |
2 | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | natural abundance | 1 mM | |
3 | MAGNESIUM ION | natural abundance | 8 mM | |
4 | sodium chloride | natural abundance | 120 mM | |
5 | sodium phosphate | natural abundance | 20 mM | |
6 | KOB | natural abundance | 3.8 mM | |
7 | D2O | natural abundance | 80 % | |
8 | DMSO_d6 | natural abundance | 20 % |
Solvent system 80% D2O/20% DMSO_d6, Pressure 1 atm, Temperature 278 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | H-RasT35S | natural abundance | 1 mM | |
10 | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | natural abundance | 1 mM | |
11 | MAGNESIUM ION | natural abundance | 8 mM | |
12 | sodium chloride | natural abundance | 120 mM | |
13 | sodium phosphate | natural abundance | 20 mM | |
14 | KOB | natural abundance | 3.8 mM | |
15 | D2O | natural abundance | 80 % | |
16 | DMSO_d6 | natural abundance | 20 % |