Resonance assignment of As-p18, a structurally unusual fatty acid binding protein secreted by developing larvae of the parasitic nematode Ascaris suum
MRGSHHHHHH GSKTLPDKFL GTFKLERDEN FDEYLKARGY GWIMRQVIKL AGVTKKFRNA ASGKPDRYDM ENLTTKKDTH HKDWALGEEF QDEALDSTQH KITFDLKDPN TLTETHIKVD DPTDVETYEY RRDGDYLVMK MSWKGVSTSR YYKKQ
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.1 % (1701 of 1868) | 90.7 % (888 of 979) | 91.3 % (664 of 727) | 92.0 % (149 of 162) |
Backbone | 92.6 % (854 of 922) | 92.1 % (291 of 316) | 93.4 % (425 of 455) | 91.4 % (138 of 151) |
Sidechain | 88.9 % (970 of 1091) | 89.0 % (590 of 663) | 88.7 % (370 of 417) | 90.9 % (10 of 11) |
Aromatic | 72.0 % (144 of 200) | 73.0 % (73 of 100) | 70.1 % (68 of 97) | 100.0 % (3 of 3) |
Methyl | 96.8 % (120 of 124) | 96.8 % (60 of 62) | 96.8 % (60 of 62) |
1. rAs-p18
MRGSHHHHHH GSKTLPDKFL GTFKLERDEN FDEYLKARGY GWIMRQVIKL AGVTKKFRNA ASGKPDRYDM ENLTTKKDTH HKDWALGEEF QDEALDSTQH KITFDLKDPN TLTETHIKVD DPTDVETYEY RRDGDYLVMK MSWKGVSTSR YYKKQSolvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400, Details pH 7.4 298K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Oleic Acid | natural abundance | 2000.0 mM | |
6 | rAs-p18 | [U-99% 13C; U-99% 15N] | 500.0 mM | |
7 | Sodium phosphate buffer | natural abundance | 20.0 mM |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.3 % (3336 of 3736) | 91.3 % (1787 of 1958) | 85.9 % (1249 of 1454) | 92.6 % (300 of 324) |
Backbone | 88.7 % (1635 of 1844) | 92.9 % (587 of 632) | 84.6 % (770 of 910) | 92.1 % (278 of 302) |
Sidechain | 90.0 % (1964 of 2182) | 90.0 % (1193 of 1326) | 89.9 % (750 of 834) | 95.5 % (21 of 22) |
Aromatic | 72.5 % (290 of 400) | 73.0 % (146 of 200) | 71.1 % (138 of 194) | 100.0 % (6 of 6) |
Methyl | 98.4 % (244 of 248) | 98.4 % (122 of 124) | 98.4 % (122 of 124) |
1. rAs-p18
MRGSHHHHHH GSKTLPDKFL GTFKLERDEN FDEYLKARGY GWIMRQVIKL AGVTKKFRNA ASGKPDRYDM ENLTTKKDTH HKDWALGEEF QDEALDSTQH KITFDLKDPN TLTETHIKVD DPTDVETYEY RRDGDYLVMK MSWKGVSTSR YYKKQSolvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.200, Details pH 7.2 298K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | rAs-p18 | [U-99% 13C; U-99% 15N] | 400.0 mM | |
4 | Sodium phosphate buffer | natural abundance | 20.0 mM |