NMR structure of RelA-TAD/CBP-TAZ1 complex
ATGPTADPEK RKLIQQQLVL LLHAHKCQRR EQANGEVRAC SLPHCRTMKN VLNHMTHCQA GKACQVAHCA SSRQIISHWK NCTRHDCPVC LPLKNASDKR
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS27:SG | 3:ZN1:ZN |
2 | na | sing | 1:CYS40:SG | 3:ZN1:ZN |
3 | na | sing | 1:CYS58:SG | 3:ZN1:ZN |
4 | na | sing | 1:CYS64:SG | 3:ZN1:ZN |
5 | na | sing | 1:CYS69:SG | 3:ZN1:ZN |
6 | na | sing | 1:CYS82:SG | 3:ZN1:ZN |
7 | na | sing | 1:CYS87:SG | 3:ZN1:ZN |
8 | na | sing | 1:CYS90:SG | 3:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 69.1 % (1304 of 1887) | 62.7 % (616 of 982) | 71.1 % (512 of 720) | 95.1 % (176 of 185) |
Backbone | 82.5 % (830 of 1006) | 93.0 % (319 of 343) | 70.8 % (354 of 500) | 96.3 % (157 of 163) |
Sidechain | 60.7 % (632 of 1041) | 46.5 % (297 of 639) | 83.2 % (316 of 380) | 86.4 % (19 of 22) |
Aromatic | 73.3 % (63 of 86) | 74.4 % (32 of 43) | 71.4 % (30 of 42) | 100.0 % (1 of 1) |
Methyl | 70.9 % (122 of 172) | 98.8 % (85 of 86) | 43.0 % (37 of 86) |
1. TAZ1
ATGPTADPEK RKLIQQQLVL LLHAHKCQRR EQANGEVRAC SLPHCRTMKN VLNHMTHCQA GKACQVAHCA SSRQIISHWK NCTRHDCPVC LPLKNASDKR2. RelA-TA2
GSHMKSTQAG EGTLSEALLH LQFDADEDLG ALLGNSTDPG VFTDLASVDN SEFQQLLNQG VSMSHSTAEPSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TAZ1 | [U-13C; U-15N] | 0.8 ~ 1.1 mM | |
2 | RelA-TA2 | natural abundance | 1.2 ~ 1.5 mM | |
3 | DTT | natural abundance | 2 mM | |
4 | TRIS | natural abundance | 20 mM | |
5 | sodium chloride | natural abundance | 40 mM | |
6 | D2O | [U-2H] | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | TAZ1 | [U-13C] | 0.8 ~ 1.1 mM | |
8 | RelA-TA2 | natural abundance | 1.2 ~ 1.5 mM | |
9 | DTT | [U-2H] | 2 mM | |
10 | TRIS | [U-2H] | 20 mM | |
11 | sodium chloride | natural abundance | 40 mM | |
12 | D2O | [U-2H] | 99.8 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | RelA-TA2 | [U-13C] | 0.7 ~ 0.9 mM | |
14 | TAZ1 | natural abundance | 1.5 ~ 1.8 mM | |
15 | DTT | [U-2H] | 2 mM | |
16 | TRIS | [U-2H] | 20 mM | |
17 | sodium chloride | natural abundance | 40 mM | |
18 | D2O | [U-2H] | 99.8 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | RelA-TA2 | [U-13C; U-15N] | 0.7 ~ 0.9 mM | |
20 | TAZ1 | natural abundance | 1.5 ~ 1.8 mM | |
21 | DTT | natural abundance | 2 mM | |
22 | TRIS | natural abundance | 20 mM | |
23 | sodium chloride | natural abundance | 40 mM | |
24 | D2O | [U-2H] | 5 % | |
25 | H2O | natural abundance | 95 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
26 | TAZ1 | [U-15N] | 0.8 ~ 1.1 mM | |
27 | RelA-TA2 | natural abundance | 1.2 ~ 1.5 mM | |
28 | DTT | natural abundance | 2 mM | |
29 | TRIS | natural abundance | 20 mM | |
30 | sodium chloride | natural abundance | 40 mM | |
31 | D2O | [U-2H] | 5 % | |
32 | H2O | natural abundance | 95 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
33 | RelA-TA2 | [U-15N] | 0.7 ~ 0.9 mM | |
34 | TAZ1 | natural abundance | 1.5 ~ 1.8 mM | |
35 | DTT | natural abundance | 2 mM | |
36 | TRIS | natural abundance | 20 mM | |
37 | sodium chloride | natural abundance | 40 mM | |
38 | D2O | [U-2H] | 5 % | |
39 | H2O | natural abundance | 95 % |