Solution Structure of Bacterial Intein-Like domain from Clostridium thermocellum
SMKAFVAGTM ILTATGLVAI ENIKAGDKVI ATNPETFEVA EKTVLETYVR ETTELLHLTI GGEVIKTTFD HPFYVKDVGF VEAGKLQVGD KLLDSRGNVL VVEEKKLEIA DKPVKVYNFK VDDFHTYHVG DNEVLVHN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.8 % (1517 of 1600) | 94.7 % (773 of 816) | 94.4 % (606 of 642) | 97.2 % (138 of 142) |
Backbone | 98.1 % (806 of 822) | 97.2 % (275 of 283) | 98.8 % (399 of 404) | 97.8 % (132 of 135) |
Sidechain | 92.5 % (838 of 906) | 93.4 % (498 of 533) | 91.3 % (334 of 366) | 85.7 % (6 of 7) |
Aromatic | 61.5 % (75 of 122) | 70.5 % (43 of 61) | 52.5 % (32 of 61) | |
Methyl | 99.0 % (198 of 200) | 99.0 % (99 of 100) | 99.0 % (99 of 100) |
1. CthBIL4
SMKAFVAGTM ILTATGLVAI ENIKAGDKVI ATNPETFEVA EKTVLETYVR ETTELLHLTI GGEVIKTTFD HPFYVKDVGF VEAGKLQVGD KLLDSRGNVL VVEEKKLEIA DKPVKVYNFK VDDFHTYHVG DNEVLVHNSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CthBIL4 | [U-99% 13C; U-99% 15N] | 1.2 mM | |
2 | potassium phosphate | natural abundance | 20 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % |