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BMRB: 18667


18667
4B6U
Solution structure of eIF4E3 in complex with m7GDP
Authors
Osborne, M.J., Osborne, M.J., Volpon, L., Kornblatt, J., Culkjovic-kraljcic, B., Borden, A.
Assembly
Solution structure of eIF4E3 in complex with m7GDP
Entity
1. EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE (polymer, Thiol state: all free), 207 monomers, 22835.81 Da Detail

MALPPAAAPP GANEPLDKAL SALPPEPGGV PLHSPWTFWL DRSLPGATAA ECASNLKKIY TVQTVQIFWS VYNNIPPVTS LPLRCSYHLM RGERRPLWEE ESNAKGGVWK MKVPKDSTST VWKELLLATI GEQFTDCAAA DDEIIGVSVS VRDREDVVQV WNVNASLVGE ATVLEKIHQL LPHIAFKAVF YKPHEEHHAF EGGRGKH


2. 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE (non-polymer, Thiol state: not present), 459.243 Da
Total weight
23295.053 Da
Max. entity weight
22835.81 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Refine. method
XPLOR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.0 %, Completeness: 89.6 %, Completeness (bb): 95.4 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All89.6 % (2145 of 2393)88.1 % (1091 of 1238)89.8 % (850 of 947)98.1 % (204 of 208)
Backbone95.4 % (1151 of 1206)96.8 % (396 of 409)93.8 % (570 of 608)97.9 % (185 of 189)
Sidechain85.6 % (1182 of 1381)83.8 % (695 of 829)87.8 % (468 of 533)100.0 % (19 of 19)
Aromatic90.9 % (189 of 208)92.3 % (96 of 104)88.7 % (86 of 97)100.0 % (7 of 7)
Methyl100.0 % (242 of 242)100.0 % (121 of 121)100.0 % (121 of 121)

1. EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE

MALPPAAAPP GANEPLDKAL SALPPEPGGV PLHSPWTFWL DRSLPGATAA ECASNLKKIY TVQTVQIFWS VYNNIPPVTS LPLRCSYHLM RGERRPLWEE ESNAKGGVWK MKVPKDSTST VWKELLLATI GEQFTDCAAA DDEIIGVSVS VRDREDVVQV WNVNASLVGE ATVLEKIHQL LPHIAFKAVF YKPHEEHHAF EGGRGKH

Show sample condition
Sample

Solvent system 90%water 10% d2o, Pressure 1 atm, Temperature 300.150 K, pH 7.200, Details 0.25


#NameIsotope labelingTypeConcentration
1EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE[U-13C; U-15N]0.25 mM
2H2Onatural abundance90 %
3D2O[U-13C; U-15N]10 %

LACS Plot; CA
Referencing offset: -0.19 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.19 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.12 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: -0.87 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 4B6U, Strand ID: A Detail

Release date
2015-07-02
Citation
eIF4E3 acts as a tumor suppressor by utilizing an atypical mode of methyl-7-guanosine cap recognition
Osborne, M.J., Volpon, L., Kornblatt, J.A., Culjkovic-kraljcic, B., Baguet, A., Borden, K.L.
Proc. Natl. Acad. Sci. U. S. A. (2013), 110, 3877-3882, PubMed 23431134 , DOI 10.1073/pnas.1216862110 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 18663 released on 2013-02-27
    Title The third member of the eIF4E family represses gene expression via a novel mode of recognition of the methyl-7 guanosine cap moiety
Related entities 1. EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE, : 1 : 3 : 65 entities Detail
More details for 69 related entities
Interaction partners 1. EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE, : 6 interactors Detail
More details for 6 interactors identified by 1 citations
Experiments performed 1 experiments Detail
nullKeywords Cap Binding, translation