BMRB: 18667

Solution structure of eIF4E3 in complex with m7GDP

Authors

Osborne, M.J., Osborne, M.J., Volpon, L., Kornblatt, J., Culkjovic-kraljcic, B., Borden, A.

Assembly

Solution structure of eIF4E3 in complex with m7GDP

Entity

1. EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE (polymer, Thiol state: all free), 207 monomers, 22835.81 Da Detail

MALPPAAAPP GANEPLDKAL SALPPEPGGV PLHSPWTFWL DRSLPGATAA ECASNLKKIY TVQTVQIFWS VYNNIPPVTS LPLRCSYHLM RGERRPLWEE ESNAKGGVWK MKVPKDSTST VWKELLLATI GEQFTDCAAA DDEIIGVSVS VRDREDVVQV WNVNASLVGE ATVLEKIHQL LPHIAFKAVF YKPHEEHHAF EGGRGKH

2. 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE (non-polymer, Thiol state: not present), 459.243 Da

Total weight

23295.053 Da

Max. entity weight

22835.81 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

XPLOR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 89.6 %, Completeness (bb): 95.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.6 % (2145 of 2393)	88.1 % (1091 of 1238)	89.8 % (850 of 947)	98.1 % (204 of 208)
Backbone	95.4 % (1151 of 1206)	96.8 % (396 of 409)	93.8 % (570 of 608)	97.9 % (185 of 189)
Sidechain	85.6 % (1182 of 1381)	83.8 % (695 of 829)	87.8 % (468 of 533)	100.0 % (19 of 19)
Aromatic	90.9 % (189 of 208)	92.3 % (96 of 104)	88.7 % (86 of 97)	100.0 % (7 of 7)
Methyl	100.0 % (242 of 242)	100.0 % (121 of 121)	100.0 % (121 of 121)

1. EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE

MALPPAAAPP GANEPLDKAL SALPPEPGGV PLHSPWTFWL DRSLPGATAA ECASNLKKIY TVQTVQIFWS VYNNIPPVTS LPLRCSYHLM RGERRPLWEE ESNAKGGVWK MKVPKDSTST VWKELLLATI GEQFTDCAAA DDEIIGVSVS VRDREDVVQV WNVNASLVGE ATVLEKIHQL LPHIAFKAVF YKPHEEHHAF EGGRGKH

Show sample condition

Sample

Solvent system 90%water 10% d2o, Pressure 1 atm, Temperature 300.150 K, pH 7.200, Details 0.25

#	Name	Isotope labeling	Type	Concentration
1	EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE	[U-13C; U-15N]		0.25 mM
2	H2O	natural abundance		90 %
3	D2O	[U-13C; U-15N]		10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0

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LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0

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LACS Plot; CO

Referencing offset: -0.87 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0

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Protein Blocks Logo

Calculated from 10 models in PDB: 4B6U, Strand ID: A Detail

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Release date

2015-07-02

Citation

eIF4E3 acts as a tumor suppressor by utilizing an atypical mode of methyl-7-guanosine cap recognition
Osborne, M.J., Volpon, L., Kornblatt, J.A., Culjkovic-kraljcic, B., Baguet, A., Borden, K.L.
Proc. Natl. Acad. Sci. U. S. A. (2013), 110, 3877-3882, PubMed 23431134 , DOI 10.1073/pnas.1216862110 , Abstract

Related entities 1. EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE, : 1 : 3 : 65 entities Detail

Interaction partners 1. EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE, : 6 interactors Detail

Experiments performed 1 experiments Detail