Solution structure of the Sgt2 homodimerization domain
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.0 % (714 of 811) | 86.5 % (359 of 415) | 89.3 % (284 of 318) | 91.0 % (71 of 78) |
Backbone | 91.9 % (408 of 444) | 92.8 % (142 of 153) | 91.7 % (199 of 217) | 90.5 % (67 of 74) |
Sidechain | 85.1 % (371 of 436) | 82.8 % (217 of 262) | 88.2 % (150 of 170) | 100.0 % (4 of 4) |
Aromatic | 86.5 % (45 of 52) | 88.5 % (23 of 26) | 84.6 % (22 of 26) | |
Methyl | 84.9 % (73 of 86) | 83.7 % (36 of 43) | 86.0 % (37 of 43) |
1. Sgt2
SVDSASKEEI AALIVNYFSS IVEKKEISED GADSLNVAMD CISEAFGFER EAVSGILGKS EFKGQHLADI LNSASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Sgt2 | [U-100% 15N] | 2 mM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | sodium azide | natural abundance | 0.02 % | |
9 | Polyacrylamide | natural abundance | 7 % | |
10 | H2O | natural abundance | 90 % | |
11 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | Sgt2 | [U-100% 15N] | 2 mM | |
13 | sodium phosphate | natural abundance | 20 mM | |
14 | sodium azide | natural abundance | 0.02 % | |
15 | H2O | natural abundance | 90 % | |
16 | D2O | natural abundance | 10 % |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | water | protons | 4.778 ppm | internal | indirect | 0.251457 |
1H | water | protons | 4.778 ppm | internal | direct | 1.0 |
15N | water | protons | 4.778 ppm | internal | indirect | 0.1013401 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | water | protons | 4.778 ppm | internal | indirect | 0.251457 |
1H | water | protons | 4.778 ppm | internal | direct | 1.0 |
15N | water | protons | 4.778 ppm | internal | indirect | 0.1013401 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | water | protons | 4.778 ppm | internal | indirect | 0.251457 |
1H | water | protons | 4.778 ppm | internal | direct | 1.0 |
15N | water | protons | 4.778 ppm | internal | indirect | 0.1013401 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | water | protons | 4.778 ppm | internal | indirect | 0.251457 |
1H | water | protons | 4.778 ppm | internal | direct | 1.0 |
15N | water | protons | 4.778 ppm | internal | indirect | 0.1013401 |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Sgt2 | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | Sgt2 | [U-100% 15N] | 2 mM | |
13 | sodium phosphate | natural abundance | 20 mM | |
14 | sodium azide | natural abundance | 0.02 % | |
15 | H2O | natural abundance | 90 % | |
16 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz
State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297.5 K, pH 6.1, Details Sample is a symmetric homodimer. 2 mM monomer, or 1 mM dimer, were used.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Sgt2 | [U-100% 15N] | 2 mM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | sodium azide | natural abundance | 0.02 % | |
9 | Polyacrylamide | natural abundance | 7 % | |
10 | H2O | natural abundance | 90 % | |
11 | D2O | natural abundance | 10 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints | combined_18670_2lxb.nef |
Input source #2: Coordindates | 2lxb.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
-0--------10--------20--------30--------40--------50--------60--------70-- SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA --------10--------20--------30--------40--------50--------60--------70----
-0--------10--------20--------30--------40--------50--------60--------70-- SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA --------10--------20--------30--------40--------50--------60--------70----
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 74 | 0 | 0 | 100.0 |
B | B | 74 | 0 | 0 | 100.0 |
Content subtype: combined_18670_2lxb.nef
Assigned chemical shifts
-0--------10--------20--------30--------40--------50--------60--------70-- SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| ||| SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQH.ADI.NSA
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
13C chemical shifts | 318 | 284 | 89.3 |
1H chemical shifts | 415 | 352 | 84.8 |
15N chemical shifts | 79 | 70 | 88.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
13C chemical shifts | 148 | 134 | 90.5 |
1H chemical shifts | 153 | 139 | 90.8 |
15N chemical shifts | 74 | 65 | 87.8 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
13C chemical shifts | 170 | 150 | 88.2 |
1H chemical shifts | 262 | 213 | 81.3 |
15N chemical shifts | 5 | 5 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
13C chemical shifts | 44 | 38 | 86.4 |
1H chemical shifts | 44 | 37 | 84.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
13C chemical shifts | 26 | 22 | 84.6 |
1H chemical shifts | 26 | 23 | 88.5 |
Distance restraints
-0--------10--------20--------30--------40--------50--------60--------70-- SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| | | ||| .VDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEF.GQH.A.I.NSA
-0--------10--------20--------30--------40--------50--------60--------70-- SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| | | ||| .VDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEF.GQH.A.I.NSA
Dihedral angle restraints
-0--------10--------20--------30--------40--------50--------60--------70-- SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||| ....ASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEF...HLADILNS -0--------10--------20--------30--------40--------50--------60--------70-
-0--------10--------20--------30--------40--------50--------60--------70-- SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||| ....ASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEF...HLADILNS -0--------10--------20--------30--------40--------50--------60--------70-
RDC restraints
-0--------10--------20--------30--------40--------50--------60--------70-- SVDSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSA |||| |||| || || |||| |||| |||| |||| | | || ||| | |||| || | | ..DSAS.EEIA....NY.SS.VEKK.ISED.ADSL.VAMD.I.E.FG.ERE.V.GILG......QH...I.N -0--------10--------20--------30--------40--------50--------60--------70