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Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, LmCsp with dT7
Authors
Lee, J., Jeong, K., Kim, Y.
Assembly
LmCsp dT7
Entity
1. LmCsp dT7 (polymer, Thiol state: not present), 66 monomers, 7265.816 Da Detail

MEQGTVKWFN AEKGFGFIER ENGDDVFVHF SAIQGDGFKS LDEGQAVTFD VEEGQRGPQA ANVQKA


Formula weight
7265.816 Da
Source organism
Listeria monocytogenes
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.5 %, Completeness: 83.4 %, Completeness (bb): 94.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All83.4 % (628 of 753)83.1 % (325 of 391)80.1 % (230 of 287)97.3 % (73 of 75)
Backbone94.4 % (372 of 394)95.0 % (133 of 140)93.1 % (176 of 189)96.9 % (63 of 65)
Sidechain74.8 % (311 of 416)76.5 % (192 of 251)70.3 % (109 of 155)100.0 % (10 of 10)
Aromatic 9.3 % (8 of 86) 9.3 % (4 of 43) 7.1 % (3 of 42)100.0 % (1 of 1)
Methyl94.2 % (49 of 52)88.5 % (23 of 26)100.0 % (26 of 26)

1. LmCsp dT7

MEQGTVKWFN AEKGFGFIER ENGDDVFVHF SAIQGDGFKS LDEGQAVTFD VEEGQRGPQA ANVQKA

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6


#NameIsotope labelingTypeConcentration
1LmCsp_dT7[U-13C; U-15N]0.8 mM
2potassium phosphatenatural abundance50 mM
3potassium chloridenatural abundance100 mM
4EDTAnatural abundance0.1 mM
5DSSnatural abundance0.004 v/v
6H2Onatural abundance90 %
7D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: -0.28 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.28 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: 0.01 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: 0.49 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2LXJ, Strand ID: A Detail


Release date
2013-08-04
Citation
Structural and dynamic features of cold-shock proteins of Listeria monocytogenes, a psychrophilic bacterium
Lee, J., Jeong, K., Jin, B., Ryu, K., Kim, E., Ahn, J., Kim, Y.
Biochemistry (2013), 52, 2492-2504, PubMed 23506337 , DOI 10.1021/bi301641b ,
Related entities 1. LmCsp dT7, : 1 : 2 : 215 entities Detail
Experiments performed 9 experiments Detail
NMR combined restraints 3 contents Detail
Keywords Nucleic acids, Protein