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BMRB: 18698

2LXU

Solution NMR Structure of the eukaryotic RNA recognition motif, RRM1, from the heterogeneous nuclear ribonucleoprotein H from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8614A

Authors

Ramelot, T.A., Yang, Y., Pederson, K., Shastry, R., Kohan, E., Janjua, H., Xiao, R., Acton, T.B., Everett, J.K., Prestegard, J.H., Montelione, G.T., Kennedy, M.A.

Assembly

HR8614A

Entity

1. HR8614A (polymer, Thiol state: all free), 108 monomers, 12151.40 Da Detail

SHMGGEGFVV KVRGLPWSCS ADEVQRFFSD CKIQNGAQGI RFIYTREGRP SGEAFVELES EDEVKLALKK DRETMGHRYV EVFKSNNVEM DWVLKHTGPN SPDTANDG

Formula weight

12151.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, conformer_family_coord_set, representative_conformer, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 96.5 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.5 % (1196 of 1240)	96.8 % (627 of 648)	95.8 % (458 of 478)	97.4 % (111 of 114)
Backbone	96.6 % (618 of 640)	96.9 % (216 of 223)	96.2 % (301 of 313)	97.1 % (101 of 104)
Sidechain	96.6 % (673 of 697)	96.7 % (411 of 425)	96.2 % (252 of 262)	100.0 % (10 of 10)
Aromatic	89.3 % (100 of 112)	91.1 % (51 of 56)	87.0 % (47 of 54)	100.0 % (2 of 2)
Methyl	98.9 % (89 of 90)	97.8 % (44 of 45)	100.0 % (45 of 45)

1. HR8614A

SHMGGEGFVV KVRGLPWSCS ADEVQRFFSD CKIQNGAQGI RFIYTREGRP SGEAFVELES EDEVKLALKK DRETMGHRYV EVFKSNNVEM DWVLKHTGPN SPDTANDG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.8 mM HR8614A.009, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	HR8614A.008	[biosynthetically directed-5% 13C; U-100% 15N]	0.8 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	calcium chloride	natural abundance	5 mM
11	DTT	natural abundance	10 mM
12	DSS	natural abundance	50 uM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 100% D2O

#	Name	Isotope labeling	Concentration
13	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
14	MES	natural abundance	20 mM
15	sodium chloride	natural abundance	100 mM
16	calcium chloride	natural abundance	5 mM
17	DTT	natural abundance	10 mM
18	DSS	natural abundance	50 uM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LXU, Strand ID: A Detail

Release date

2012-10-28

Related entities 1. HR8614A, : 1 : 7 : 118 entities Detail

Interaction partners 1. HR8614A, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 23 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

3 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

5 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

6 3D 1H-13C arom NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

7 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

9 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

10 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

11 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

12 3D (H)CCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 100% D2O

#	Name	Isotope labeling	Concentration
13	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
14	MES	natural abundance	20 mM
15	sodium chloride	natural abundance	100 mM
16	calcium chloride	natural abundance	5 mM
17	DTT	natural abundance	10 mM
18	DSS	natural abundance	50 uM

13 3D NUS 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 100% D2O

#	Name	Isotope labeling	Concentration
13	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
14	MES	natural abundance	20 mM
15	sodium chloride	natural abundance	100 mM
16	calcium chloride	natural abundance	5 mM
17	DTT	natural abundance	10 mM
18	DSS	natural abundance	50 uM

14 2D 1H-15N HSQC NH2only

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

15 4D 13C-13C-HMQC-NOESY-HMQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 100% D2O

#	Name	Isotope labeling	Concentration
13	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
14	MES	natural abundance	20 mM
15	sodium chloride	natural abundance	100 mM
16	calcium chloride	natural abundance	5 mM
17	DTT	natural abundance	10 mM
18	DSS	natural abundance	50 uM

16 1D T1 Nhsqc array

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

17 1D T2 Nhsqc array

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

18 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.8 mM HR8614A.009, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	HR8614A.008	[biosynthetically directed-5% 13C; U-100% 15N]	0.8 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	calcium chloride	natural abundance	5 mM
11	DTT	natural abundance	10 mM
12	DSS	natural abundance	50 uM

19 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.8 mM HR8614A.009, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	HR8614A.008	[biosynthetically directed-5% 13C; U-100% 15N]	0.8 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	calcium chloride	natural abundance	5 mM
11	DTT	natural abundance	10 mM
12	DSS	natural abundance	50 uM

20 2D 1H-13C HSQC arom CT

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

21 2D 1H-13C HSQC arom noCT

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM HR8614A.007, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8614A.007	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	DSS	natural abundance	50 uM

22 2D 1H-15N HSQC_His

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.8 mM HR8614A.009, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	HR8614A.008	[biosynthetically directed-5% 13C; U-100% 15N]	0.8 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	calcium chloride	natural abundance	5 mM
11	DTT	natural abundance	10 mM
12	DSS	natural abundance	50 uM

23 2D 1H-15N heteronuclear NOE interleaved

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.8 mM HR8614A.009, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	HR8614A.008	[biosynthetically directed-5% 13C; U-100% 15N]	0.8 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	calcium chloride	natural abundance	5 mM
11	DTT	natural abundance	10 mM
12	DSS	natural abundance	50 uM

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18698_2lxu.nef
Input source #2: Coordindates	2lxu.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-----10--------20--------30--------40--------50--------60--------70--------80--------90-------100---
SHMGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPN
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SHMGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPN
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

----110-
SPDTANDG
||||||||
SPDTANDG
--------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	108	0	0	100.0

Content subtype: combined_18698_2lxu.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1208		98.1 (chain: A, length: 108)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	1754 (1)	noe	96.3 (chain: A, length: 108)
2	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	80 (1)	hbond	56.5 (chain: A, length: 108)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	102 (102)	.	64.8 (chain: A, length: 108)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 108, Sequence coverage: 98.1%
- Total number of assignments
  - ¹H chemical shifts: 629
  - ¹³C chemical shifts: 466
  - ¹⁵N chemical shifts: 113
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
28	GLN	CD	179.1
37	GLN	CD	179.6
38	ASN	CG	178.3
41	GLN	CD	180.4
89	ASN	CG	177.3
90	ASN	CG	175.3
103	ASN	CG	177.3
109	ASN	CG	177.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	648	629	97.1
¹³C chemical shifts	478	458	95.8
¹⁵N chemical shifts	121	113	93.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	223	217	97.3
¹³C chemical shifts	216	206	95.4
¹⁵N chemical shifts	104	100	96.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	425	412	96.9
¹³C chemical shifts	262	252	96.2
¹⁵N chemical shifts	17	13	76.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	47	97.9
¹³C chemical shifts	48	47	97.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	56	51	91.1
¹³C chemical shifts	54	47	87.0
¹⁵N chemical shifts	2	2	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 108, Sequence coverage: 96.3%
- Total number of restraints: 1750
- Weight of restraints: 1.0 (1750)
- Potential type of restraints: square-well-parabolic (1750)
- Range of distance target values: 2.25, 3.9 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_2
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 108, Sequence coverage: 56.5%
  - Total number of restraints: 80
  - Weight of restraints: 1.0 (80)
  - Potential type of restraints: square-well-parabolic (80)
  - Range of distance target values: 2.05, 3.0 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 108, Sequence coverage: 64.8%
- Total number of restraints: 102
- Total number of restraints per polymer type: 102
- Weight of restraints: 1.0 (102)
- Potential type of restraints: square-well-parabolic (102)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords HNRH1_HUMAN, HNRNPH1, HNRPH, HNRPH1, RRM1, Target HR8614A, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG), PSI-Biology, Protein NMR, Protein Structure Initiative, RRM, Structural Genomics

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Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.1 seconds)

BMRB: 18698

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. HR8614A, : 1 : 7 : 118 entities Detail

Interaction partners 1. HR8614A, : 3 interactors Detail

Experiments performed 23 experiments Detail

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