BMRBj - BMREntryMaster

Found 1 Document (0.607 seconds)

BMRB: 18701

2LXX

Solution struture of cofilin like UNC-60B protein from Caenorhabditis elegans

Authors

SHUKLA, V., YADAV, R., KABRA, A., JAIN, A., KUMAR, D., ONO, S., ARORA, A.

Assembly

UNC-60B from Caenorhabditis elegans

Entity

1. UNC-60B from Caenorhabditis elegans (polymer), 152 monomers, 17046.25 Da Detail

MASGVKVDPS CKNAYDLLHN KHQHSYIIFK IDKNDTAIVV EKVGEKNAPY AEFVEEMKKL VEDGKECRYA AVDVEVTVQR QGAEGTSTLN KVIFVQYCPD NAPVRRRMLY ASSVRALKAS LGLESLFQVQ ASEMSDLDEK SVKSDLMSNQ RI

Formula weight

17046.25 Da

Source organism

Caenorhabditis elegans

Exptl. method

solution NMR

Refine. method

Water refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 92.3 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.3 % (1627 of 1763)	91.0 % (836 of 919)	95.0 % (648 of 682)	88.3 % (143 of 162)
Backbone	96.2 % (870 of 904)	95.8 % (293 of 306)	96.7 % (435 of 450)	95.9 % (142 of 148)
Sidechain	87.5 % (879 of 1005)	86.6 % (531 of 613)	91.8 % (347 of 378)	7.1 % (1 of 14)
Aromatic	83.0 % (83 of 100)	82.0 % (41 of 50)	84.0 % (42 of 50)
Methyl	98.8 % (168 of 170)	100.0 % (85 of 85)	97.6 % (83 of 85)

1. entity

MASGVKVDPS CKNAYDLLHN KHQHSYIIFK IDKNDTAIVV EKVGEKNAPY AEFVEEMKKL VEDGKECRYA AVDVEVTVQR QGAEGTSTLN KVIFVQYCPD NAPVRRRMLY ASSVRALKAS LGLESLFQVQ ASEMSDLDEK SVKSDLMSNQ RI

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N LABELED UNC-60B

#	Name	Isotope labeling	Concentration
1	UNC-60B	[U-99% 15N]	0.9 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.1 %
5	H2O	natural abundance	93 %
6	D2O	natural abundance	7 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
13	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.1 %
17	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LXX, Strand ID: A Detail

Release date

2013-10-07

Citation

Solution structures and dynamics of ADF/cofilins UNC-60A and UNC-60B from Caenorhabditis elegans
SHUKLA, V., YADAV, R., KABRA, A., JAIN, A., KUMAR, D., ONO, S., ARORA, A.
Biochem. J. (2015), 465, 63-78, PubMed 25279657 , DOI 10.1042/BJ20140923 , Abstract

Related entities 1. UNC-60B from Caenorhabditis elegans, : 1 : 1 : 158 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N LABELED UNC-60B

#	Name	Isotope labeling	Concentration
1	UNC-60B	[U-99% 15N]	0.9 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.1 %
5	H2O	natural abundance	93 %
6	D2O	natural abundance	7 %

2 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

4 3D H(CCO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

5 3D C(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

6 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

7 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

8 3D HNCO-NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

9 3D HN(CA)CO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

10 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

11 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
13	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.1 %
17	D2O	natural abundance	100 %

12 CB(CGCD)HD

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

13 CB(CGCDCE)HE

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
7	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	sodium azide	natural abundance	0.1 %
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %

14 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N LABELED UNC-60B

#	Name	Isotope labeling	Concentration
1	UNC-60B	[U-99% 15N]	0.9 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.1 %
5	H2O	natural abundance	93 %
6	D2O	natural abundance	7 %

15 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
13	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.1 %
17	D2O	natural abundance	100 %

16 2D 1H-13C HSQC aromatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
13	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.1 %
17	D2O	natural abundance	100 %

17 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N 13C labeled UNC60B

#	Name	Isotope labeling	Concentration
13	UNC-60B	[U-99% 13C; U-99% 15N]	0.9 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	sodium azide	natural abundance	0.1 %
17	D2O	natural abundance	100 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr18701_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr18701_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1795		98.7 (chain: 1, length: 152)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 152, Sequence coverage: 98.7%
- Total number of assignments
  - ¹H chemical shifts: 1005
  - ¹³C chemical shifts: 646
  - ¹⁵N chemical shifts: 144
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	919	835	90.9
¹³C chemical shifts	682	646	94.7
¹⁵N chemical shifts	169	144	85.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	306	299	97.7
¹³C chemical shifts	304	297	97.7
¹⁵N chemical shifts	148	144	97.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	613	536	87.4
¹³C chemical shifts	378	349	92.3
¹⁵N chemical shifts	21	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	90	85	94.4
¹³C chemical shifts	90	83	92.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	42	84.0
¹³C chemical shifts	50	42	84.0

Keywords ADF/Cofilin fold

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Found 1 Document (0.607 seconds)

BMRB: 18701

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. UNC-60B from Caenorhabditis elegans, : 1 : 1 : 158 entities Detail

Experiments performed 17 experiments Detail

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Chemical shift validation 3 contents Detail