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BMRB: 18735

2LZ1

Solution NMR Structure of the DNA-Binding Domain of Human NF-E2-Related Factor 2, Northeast Structural Genomics Consortium (NESG) Target HR3520O

Authors

Eletsky, A., Pulavarti, S.V.S.R.K., Lee, D., Kohan, E., Janjua, H., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.T., Szyperski, T.

Assembly

HR3520O

Entity

1. HR3520O (polymer, Thiol state: all free), 90 monomers, 10640.23 Da Detail

MGHHHHHHSH MKHSSRLEAH LTRDELRAKA LHIPFPVEKI INLPVVDFNE MMSKEQFNEA QLALIRDIRR RGKNKVAAQN CRKRKLENIV

Formula weight

10640.23 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 92.2 %, Completeness: 86.4 %, Completeness (bb): 90.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.4 % (953 of 1103)	86.2 % (504 of 585)	86.5 % (365 of 422)	87.5 % (84 of 96)
Backbone	90.4 % (483 of 534)	89.9 % (161 of 179)	91.0 % (244 of 268)	89.7 % (78 of 87)
Sidechain	84.0 % (552 of 657)	84.5 % (343 of 406)	83.9 % (203 of 242)	66.7 % (6 of 9)
Aromatic	45.7 % (32 of 70)	45.7 % (16 of 35)	45.7 % (16 of 35)
Methyl	95.7 % (88 of 92)	95.7 % (44 of 46)	95.7 % (44 of 46)

1. HR3520O

MGHHHHHHSH MKHSSRLEAH LTRDELRAKA LHIPFPVEKI INLPVVDFNE MMSKEQFNEA QLALIRDIRR RGKNKVAAQN CRKRKLENIV

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.5 mM HR3520O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR3520O	[U-100% 13C; U-100% 15N]	0.5 mM
2	DSS	natural abundance	50 uM
3	sodium azide	natural abundance	0.02 %
4	Arginine	natural abundance	50 mM
5	DTT	natural abundance	10 mM
6	ammonium acetate	natural abundance	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.5 mM HR3520O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
7	HR3520O	[5% 13C; U-100% 15N]	0.5 mM
8	DSS	natural abundance	50 uM
9	sodium azide	natural abundance	0.02 %
10	Arginine	natural abundance	50 mM
11	DTT	natural abundance	10 mM
12	ammonium acetate	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LZ1, Strand ID: A Detail

Release date

2012-11-20

Related entities 1. HR3520O, : 1 : 1 : 32 entities Detail

Interaction partners 1. HR3520O, : 24 interactors Detail

More details for 24 interactors identified by 13 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC