FGFR3tm
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.8 % (452 of 482) | 93.8 % (226 of 241) | 93.0 % (185 of 199) | 97.6 % (41 of 42) |
Backbone | 96.1 % (246 of 256) | 94.4 % (84 of 89) | 96.8 % (121 of 125) | 97.6 % (41 of 42) |
Sidechain | 92.5 % (245 of 265) | 93.4 % (142 of 152) | 91.2 % (103 of 113) | |
Aromatic | 78.3 % (36 of 46) | 100.0 % (23 of 23) | 56.5 % (13 of 23) | |
Methyl | 100.0 % (74 of 74) | 100.0 % (37 of 37) | 100.0 % (37 of 37) |
1. FGFR3tm
LPAEEELVEA DEAGSVYAGI LSYGVGFFLF ILVVAAVTLC RLRSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FGFR3tm | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | FGFR3tm | natural abundance | 0.75 mM | |
3 | DPC | [U-99% 2H] | 88 mM | |
4 | SDS | [U-99% 2H] | 10 mM | |
5 | sodium azide | natural abundance | 0.3 mM | |
6 | TCEP | natural abundance | 6 mM | |
7 | citric acid | natural abundance | 5 mM | |
8 | Na2HPO4 | natural abundance | 15 mM | |
9 | H2O | natural abundance | 95 % | |
10 | D2O | natural abundance | 5 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints | combined_18763_2lzl.nef |
Input source #2: Coordindates | 2lzl.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
---360-----370-------380-------390--------- LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR ||||||||||||||||||||||||||||||||||||||||||| LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR --------10--------20--------30--------40---
---360-----370-------380-------390--------- LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR ||||||||||||||||||||||||||||||||||||||||||| LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR --------10--------20--------30--------40---
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 43 | 0 | 0 | 100.0 |
B | B | 43 | 0 | 0 | 100.0 |
Content subtype: combined_18763_2lzl.nef
Assigned chemical shifts
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
397 | ARG | HH11 | 6.886 |
397 | ARG | HH12 | 6.886 |
397 | ARG | HH21 | 6.908 |
397 | ARG | HH22 | 6.908 |
397 | ARG | CZ | 159.35 |
397 | ARG | NH1 | 72.241 |
397 | ARG | NH2 | 72.244 |
399 | ARG | HH11 | 6.814 |
399 | ARG | HH12 | 6.814 |
399 | ARG | HH21 | 6.835 |
399 | ARG | HH22 | 6.835 |
399 | ARG | CZ | 159.267 |
399 | ARG | NH1 | 72.266 |
399 | ARG | NH2 | 72.302 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 241 | 240 | 99.6 |
13C chemical shifts | 199 | 187 | 94.0 |
15N chemical shifts | 44 | 43 | 97.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 89 | 88 | 98.9 |
13C chemical shifts | 86 | 84 | 97.7 |
15N chemical shifts | 42 | 41 | 97.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 152 | 152 | 100.0 |
13C chemical shifts | 113 | 103 | 91.2 |
15N chemical shifts | 2 | 2 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 37 | 37 | 100.0 |
13C chemical shifts | 37 | 37 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 23 | 23 | 100.0 |
13C chemical shifts | 23 | 13 | 56.5 |
Distance restraints