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BMRB: 18791

Sequence-specific backbone 1H, 13C and 15N assignments of the catalytic domain of the Escherichia coli protein tyrosine kinase, Wzc

Authors

Temel, D., Dutta, K., Ghose, R.

Assembly

WzcCD

Entity

1. WzcCD (polymer, Thiol state: all free), 295 monomers, 32533.91 Da Detail

MGSSHHHHHH SSGLVPRGSH MSLFNRGIES PQVLEEHGIS VYASIPLSEW QKARDSVKTI KGIKRYKQSQ LLAVGNPTDL AIEAIRSLRT SLHFAMMQAQ NNVLMMTGVS PSIGKTFVCA NLAAVISQTN KRVLLIDCDM RKGYTHELLG TNNVNGLSEI LIGQGDITTA AKPTSIAKFD LIPRGQVPPN PSELLMSERF AELVNWASKN YDLVLIDTPP ILAVTDAAIV GRHVGTTLMV ARYAVNTLKE VETSLSRFEQ NGIPVKGVIL NSIFRRASAY QDYGYYEYEY KSDAK

Formula weight

32533.91 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.5 %, Completeness: 39.0 %, Completeness (bb): 70.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	39.0 % (1322 of 3393)	19.1 % (335 of 1754)	56.2 % (747 of 1329)	77.4 % (240 of 310)
Backbone	70.9 % (1236 of 1744)	44.7 % (267 of 597)	84.3 % (729 of 865)	85.1 % (240 of 282)
Sidechain	16.1 % (310 of 1924)	6.0 % (69 of 1157)	32.6 % (241 of 739)	0.0 % (0 of 28)
Aromatic	0.0 % (0 of 226)	0.0 % (0 of 113)	0.0 % (0 of 111)	0.0 % (0 of 2)
Methyl	12.4 % (45 of 364)	7.1 % (13 of 182)	17.6 % (32 of 182)

1. WzcCD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	WzcCD	[U-15N; U-2H]	300 uM
2	D20	natural abundance	10 %
3	H20	natural abundance	90 %
4	Sodium phosphate	natural abundance	50 mM
5	NaCl	natural abundance	50 mM
6	DTT	natural abundance	25 mM
7	EDTA	natural abundance	5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	WzcCD	[U-13C; U-15N; U-2H]	300 uM
9	D20	natural abundance	10 %
10	H20	natural abundance	90 %
11	Sodium phosphate	natural abundance	50 mM
12	NaCl	natural abundance	50 mM
13	DTT	natural abundance	25 mM
14	EDTA	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	na	indirect	0.2514495
¹H	water	protons	4.75 ppm	na	direct	1.0
¹⁵N	water	protons	4.75 ppm	na	indirect	0.101329

Referencing offset: 0.67 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	na	indirect	0.2514495
¹H	water	protons	4.75 ppm	na	direct	1.0
¹⁵N	water	protons	4.75 ppm	na	indirect	0.101329

Referencing offset: 0.67 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	na	indirect	0.2514495
¹H	water	protons	4.75 ppm	na	direct	1.0
¹⁵N	water	protons	4.75 ppm	na	indirect	0.101329

Referencing offset: 0.4 ppm, Outliers: 1 Detail

Release date

2013-01-28

Citation

Sequence-specific backbone ¹H, ¹³C and ¹⁵N assignments of the catalytic domain of the Escherichia coli protein tyrosine kinase, Wzc
Temel, D.B., Dutta, K., Ghose, R.
Biomol. NMR Assign. (2014), 8, 37-41, PubMed 23225198 , DOI 10.1007/s12104-012-9448-0 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18784 released on 2013-10-20
Title SOLUTION NMR STRUCTURE OF ASTEROPSIN C FROM A MARINE SPONGE ASTEROPUS SP.

Related entities 1. WzcCD, : 3 : 23 entities Detail

Interaction partners 1. WzcCD, : 6 interactors Detail

More details for 6 interactors identified by 4 citations

Experiments performed 7 experiments Detail

1 3D HNCACB