NMR structure of OmpX in DPC micelles
ATSTVTGGYA QSDAQGQMNK MGGFNLKYRY EEDNSPLGVI GSFTYTEKSR TASSGDYNKN QYYGITAGPA YRINDWASIY GVVGVGYGKF QTTEYPTYKH DTSDYGFSYG AGLQFNPMEN VALDFSYEQS RIRSVDVGTW IAGVGYRF
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 43.5 % (726 of 1670) | 22.8 % (195 of 857) | 61.2 % (399 of 652) | 82.0 % (132 of 161) |
Backbone | 75.8 % (667 of 880) | 46.8 % (146 of 312) | 92.0 % (390 of 424) | 91.0 % (131 of 144) |
Sidechain | 19.4 % (178 of 918) | 9.0 % (49 of 545) | 36.0 % (128 of 356) | 5.9 % (1 of 17) |
Aromatic | 5.3 % (12 of 226) | 8.8 % (10 of 113) | 1.8 % (2 of 111) | 0.0 % (0 of 2) |
Methyl | 10.0 % (12 of 120) | 1.7 % (1 of 60) | 18.3 % (11 of 60) |
1. OmpX
ATSTVTGGYA QSDAQGQMNK MGGFNLKYRY EEDNSPLGVI GSFTYTEKSR TASSGDYNKN QYYGITAGPA YRINDWASIY GVVGVGYGKF QTTEYPTYKH DTSDYGFSYG AGLQFNPMEN VALDFSYEQS RIRSVDVGTW IAGVGYRFSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | potassium phosphate | natural abundance | 20 mM | |
2 | sodium chloride | natural abundance | 50 mM | |
3 | EDTA | natural abundance | 10 mM | |
4 | sodium azide | natural abundance | 0.05 % | |
5 | DPC | natural abundance | 110 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % | |
8 | OmpX | [U-13C; U-15N; U-2H] | 0.5 mM |