NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and Rad4
PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADGNSS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 78.2 % (1409 of 1801) | 76.8 % (720 of 937) | 78.8 % (547 of 694) | 83.5 % (142 of 170) |
Backbone | 80.9 % (773 of 956) | 80.4 % (262 of 326) | 80.6 % (382 of 474) | 82.7 % (129 of 156) |
Sidechain | 75.9 % (757 of 997) | 75.0 % (458 of 611) | 76.9 % (286 of 372) | 92.9 % (13 of 14) |
Aromatic | 92.5 % (74 of 80) | 95.0 % (38 of 40) | 89.7 % (35 of 39) | 100.0 % (1 of 1) |
Methyl | 77.8 % (126 of 162) | 76.5 % (62 of 81) | 79.0 % (64 of 81) |
1. Tfb1
PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADGNSS2. Rad4
GSTDDSVEEI QSSEEDYDSE EFEDVTDGNE VAGVEDISVE IKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Tfb1 | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | Rad4 | natural abundance | 1.25 mM | |
3 | sodium phosphate | natural abundance | 20 mM | |
4 | EDTA | natural abundance | 1 mM | |
5 | DTT | natural abundance | 1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Tfb1 | [U-100% 13C; U-100% 15N] | 1 mM | |
7 | Rad4 | natural abundance | 1.25 mM | |
8 | sodium phosphate | natural abundance | 20 mM | |
9 | EDTA | natural abundance | 1 mM | |
10 | DTT | natural abundance | 1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | Tfb1 | [U-100% 15N] | 1 mM | |
12 | Rad4 | natural abundance | 1.25 mM | |
13 | sodium phosphate | natural abundance | 20 mM | |
14 | EDTA | natural abundance | 1 mM | |
15 | DTT | natural abundance | 1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | Tfb1 | natural abundance | 1.25 mM | |
17 | Rad4 | [U-100% 13C; U-100% 15N] | 1 mM | |
18 | sodium phosphate | natural abundance | 20 mM | |
19 | EDTA | natural abundance | 1 mM | |
20 | DTT | natural abundance | 1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | Tfb1 | natural abundance | 1.25 mM | |
22 | Rad4 | [U-100% 13C; U-100% 15N] | 1 mM | |
23 | sodium phosphate | natural abundance | 20 mM | |
24 | EDTA | natural abundance | 1 mM | |
25 | DTT | natural abundance | 1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
26 | Tfb1 | natural abundance | 1.25 mM | |
27 | Rad4 | [U-100% 15N] | 1 mM | |
28 | sodium phosphate | natural abundance | 20 mM | |
29 | EDTA | natural abundance | 1 mM | |
30 | DTT | natural abundance | 1 mM |