BMRBj - BMREntryMaster

Found 1 Document (0.466 seconds)

BMRB: 18851

2M1A

HIV-1 Rev ARM peptide (residues T34-R50)

Authors

Casu, F., Duggan, B.M., Hennig, M.

Assembly

HIV-1 Rev ARM single polypeptide chain

Entity

1. HIV-1 Rev ARM single polypeptide chain (polymer, Thiol state: not present), 26 monomers, 3208.638 Da Detail

GAMATRQARR NRRRRWRERQ RAAAAR

Formula weight

3208.638 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 89.4 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.4 % (279 of 312)	86.6 % (149 of 172)	93.6 % (103 of 110)	90.0 % (27 of 30)
Backbone	92.9 % (145 of 156)	90.6 % (48 of 53)	94.8 % (73 of 77)	92.3 % (24 of 26)
Sidechain	87.8 % (159 of 181)	84.9 % (101 of 119)	94.8 % (55 of 58)	75.0 % (3 of 4)
Aromatic	83.3 % (10 of 12)	83.3 % (5 of 6)	100.0 % (5 of 5)	0.0 % (0 of 1)
Methyl	93.8 % (15 of 16)	87.5 % (7 of 8)	100.0 % (8 of 8)

1. HIV-1 Rev ARM

GAMATRQARR NRRRRWRERQ RAAAAR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N-labeled sample

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 Rev ARM	[U-15N]		1.0 mM
2	sodium phosphate	natural abundance		50 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	DTT	natural abundance		1 mM
6	sodium azide	natural abundance		0.02 %
7	D2O	[U-99% 2H]		10.0 %
8	H2O	natural abundance		90.0 %

Sample #2

Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

Release date

2013-09-03

Citation

The arginine-rich RNA-binding motif of HIV-1 Rev is intrinsically disordered and folds upon RRE binding
Casu, F., Duggan, B.M., Hennig, M.
Biophys. J. (2013), 105, 1004-1017, PubMed 23972852 , DOI 10.1016/j.bpj.2013.07.022 , Abstract

Related entities 1. HIV-1 Rev ARM single polypeptide chain, : 1 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N-labeled sample

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 Rev ARM	[U-15N]		1.0 mM
2	sodium phosphate	natural abundance		50 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	DTT	natural abundance		1 mM
6	sodium azide	natural abundance		0.02 %
7	D2O	[U-99% 2H]		10.0 %
8	H2O	natural abundance		90.0 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

3 3D HNCO

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

4 3D HNCA

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

5 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

7 3D HNCACB

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

8 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

9 3D H(CCO)NH

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

10 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N-labeled sample

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 Rev ARM	[U-15N]		1.0 mM
2	sodium phosphate	natural abundance		50 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	DTT	natural abundance		1 mM
6	sodium azide	natural abundance		0.02 %
7	D2O	[U-99% 2H]		10.0 %
8	H2O	natural abundance		90.0 %

11 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

12 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N,13C-labeled sample

#	Name	Isotope labeling	Type	Concentration
9	HIV-1 Rev ARM	[U-13C; U-15N]		1.0 mM
10	sodium phosphate	natural abundance		50 mM
11	potassium chloride	natural abundance		150 mM
12	EDTA	natural abundance		1 mM
13	DTT	natural abundance		1 mM
14	sodium azide	natural abundance		0.02 %
15	D2O	[U-99% 2H]		10.0 %
16	H2O	natural abundance		90.0 %

13 3D HNHA

Instrument

Bruker Avance - 800 MHz Equipped with a 5mm 1H[13C/15N] triple resonance probe featuring cryogenically cooled preamplifiers and r.f. coils on 1H and 13C channels and z-axis gradient

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 283 K, pH 7.4, Details Uniformly 15N-labeled sample

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 Rev ARM	[U-15N]		1.0 mM
2	sodium phosphate	natural abundance		50 mM
3	potassium chloride	natural abundance		150 mM
4	EDTA	natural abundance		1 mM
5	DTT	natural abundance		1 mM
6	sodium azide	natural abundance		0.02 %
7	D2O	[U-99% 2H]		10.0 %
8	H2O	natural abundance		90.0 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr18851_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr18851_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	293		96.2 (chain: 1, length: 26)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 26, Sequence coverage: 96.2%
- Total number of assignments
  - ¹³C chemical shifts: 103
  - ¹H chemical shifts: 163
  - ¹⁵N chemical shifts: 27
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1