BMRB: 18862

Parallel human telomeric quadruplex containing 2'F-ANA substitutions

Authors

Martin-Pintado, N., Yahyaee-Anzahaee, M., Portella, G., Orozco, M., Damha, M.J., Gonzalez, C.

Assembly

Parallel human telomeric quadruplex containing 2'F-ANA substitutions

Entity

1. Parallel human telomeric quadruplex containing 2'F-ANA substitutions (polymer, Thiol state: not present), 12 monomers, 3854.425 × 2 Da Detail

TAGGGTTAXG GT

Total weight

7708.85 Da

Max. entity weight

3854.425 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.7 %, Completeness: 92.2 %, Completeness (bb): 94.8 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	92.2 % (95 of 103)	92.2 % (95 of 103)
Suger, PO4	94.8 % (73 of 77)	94.8 % (73 of 77)
Nucleobase	84.6 % (22 of 26)	84.6 % (22 of 26)
Aromatic	81.8 % (18 of 22)	81.8 % (18 of 22)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*(GFL)P*GP*GP*T)-3')

TAGGGTTAXG GT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*(GFL)P*GP*GP*T)-3')	natural abundance		0.7 mM
2	potassium chloride	natural abundance		5 mM
3	potassium phosphate	natural abundance		10 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*(GFL)P*GP*GP*T)-3')	natural abundance		0.7 mM
5	potassium chloride	natural abundance		10 mM
6	potassium phosphate	natural abundance		5 mM

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Release date

2013-05-05

Citation

Dramatic effect of furanose C2' substitution on structure and stability: directing the folding of the human telomeric quadruplex with a single fluorine atom
Martin-Pintado, N., Yahyaee-Anzahaee, M., Deleavey, G.F., Portella, G., Orozco, M., Damha, M.J., Gonzalez, C.
J. Am. Chem. Soc. (2013), 135, 5344-5347, PubMed 23521511 , DOI 10.1021/ja401954t , Abstract

Experiments performed 4 experiments Detail