ID3 stem
Polymer type: polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 58.6 % (126 of 215) | 54.9 % (67 of 122) | 63.4 % (59 of 93) |
Suger, PO4 | 52.6 % (81 of 154) | 52.4 % (44 of 84) | 52.9 % (37 of 70) |
Nucleobase | 73.8 % (45 of 61) | 60.5 % (23 of 38) | 95.7 % (22 of 23) |
Aromatic | 84.9 % (45 of 53) | 76.7 % (23 of 30) | 95.7 % (22 of 23) |
1. RNA GGGUGUA
GGGUGUA2. RNA AGCACCC
AGCACCCSolvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA_GGGUGUA | natural abundance | 1 mM | |
2 | RNA_AGCACCC | natural abundance | 1 mM | |
3 | sodium chloride | natural abundance | 80 mM | |
4 | EDTA | natural abundance | 0.1 mM |
Bruker Avance - 600 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA_GGGUGUA | natural abundance | 1 mM | |
2 | RNA_AGCACCC | natural abundance | 1 mM | |
3 | sodium chloride | natural abundance | 80 mM | |
4 | EDTA | natural abundance | 0.1 mM |
Bruker Avance - 600 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA_GGGUGUA | natural abundance | 1 mM | |
2 | RNA_AGCACCC | natural abundance | 1 mM | |
3 | sodium chloride | natural abundance | 80 mM | |
4 | EDTA | natural abundance | 0.1 mM |
Bruker Avance - 600 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA_GGGUGUA | natural abundance | 1 mM | |
2 | RNA_AGCACCC | natural abundance | 1 mM | |
3 | sodium chloride | natural abundance | 80 mM | |
4 | EDTA | natural abundance | 0.1 mM |
Bruker Avance - 600 MHz
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA_GGGUGUA | natural abundance | 1 mM | |
2 | RNA_AGCACCC | natural abundance | 1 mM | |
3 | sodium chloride | natural abundance | 80 mM | |
4 | EDTA | natural abundance | 0.1 mM |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints | combined_18872_2m1o.nef |
Input source #2: Coordindates | 2m1o.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
------- GGGUGUA ||||||| GGGUGUA
--20--- AGCACCC ||||||| AGCACCC -------
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 7 | 0 | 0 | 100.0 |
B | B | 7 | 0 | 0 | 100.0 |
Content subtype: combined_18872_2m1o.nef
# | Content subtype | Saveframe | Status | # of rows (sets) | Experiment type | Sequence coverage (%) |
---|---|---|---|---|---|---|
1 | Assigned chemical shifts | assigned_chem_shift_list_1 | OK | 132 | 100.0 (chain: A, length: 7), 100.0 (chain: B, length: 7) | |
1 | Distance restraints | CNS/XPLOR_distance_constraints_2 | OK | 94 (1) | noe | 100.0 (chain: A, length: 7), 100.0 (chain: B, length: 7) |
Assigned chemical shifts
------- GGGUGUA ||||||| GGGUGUA
--20--- AGCACCC ||||||| AGCACCC
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 58 | 37 | 63.8 |
13C chemical shifts | 45 | 30 | 66.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 42 | 27 | 64.3 |
13C chemical shifts | 35 | 20 | 57.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 16 | 10 | 62.5 |
13C chemical shifts | 10 | 10 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 6 | 6 | 100.0 |
13C chemical shifts | 6 | 6 | 100.0 |
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
17 | A | HO2' | 4.964 |
19 | C | HO2' | 4.367 |
20 | A | HO2' | 4.583 |
21 | C | HO2' | 4.24 |
22 | C | HO2' | 4.287 |
23 | C | HO2' | 4.047 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 64 | 30 | 46.9 |
13C chemical shifts | 48 | 29 | 60.4 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 42 | 17 | 40.5 |
13C chemical shifts | 35 | 17 | 48.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 22 | 13 | 59.1 |
13C chemical shifts | 13 | 12 | 92.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 5 | 5 | 100.0 |
13C chemical shifts | 5 | 5 | 100.0 |
Distance restraints
------- GGGUGUA ||||||| GGGUGUA
--20--- AGCACCC ||||||| AGCACCC