PHD domain of ING4 N214D mutant
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 1:CYS26:SG | 2:ZN1:ZN |
2 | metal coordination | sing | 1:CYS31:SG | 2:ZN1:ZN |
3 | metal coordination | sing | 1:CYS53:SG | 2:ZN1:ZN |
4 | metal coordination | sing | 1:CYS56:SG | 2:ZN1:ZN |
5 | metal coordination | sing | 1:CYS13:SG | 2:ZN1:ZN |
6 | metal coordination | sing | 1:CYS15:SG | 2:ZN1:ZN |
7 | metal coordination | sing | 1:CYS40:SG | 2:ZN1:ZN |
8 | metal coordination | sing | 1:HIS37:ND1 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 85.2 % (386 of 453) | 83.1 % (325 of 391) | 98.4 % (61 of 62) |
Backbone | 98.9 % (179 of 181) | 99.2 % (123 of 124) | 98.2 % (56 of 57) |
Sidechain | 76.1 % (207 of 272) | 75.7 % (202 of 267) | 100.0 % (5 of 5) |
Aromatic | 63.4 % (26 of 41) | 61.5 % (24 of 39) | 100.0 % (2 of 2) |
Methyl | 100.0 % (18 of 18) | 100.0 % (18 of 18) |
1. ING4 PHD mutant N214D
MDMPVDPNEP TYCLCHQVSY GEMIGCDDPD CSIEWFHFAC VGLTTKPRGK WFCPRCSQER KKKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1), Details 0.5 mL of protein sample in 5mm NMR tube at 25 C
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ING4 PHD mutant N214D | [U-100% 15N] | 0.5 (±0.05) mM | |
2 | DSS | natural abundance | 30 uM | |
3 | DTT | natural abundance | 1 (±0.1) mM | |
4 | sodium phosphate | natural abundance | 20 (±2.0) mM | |
5 | sodium chloride | natural abundance | 50 (±5.0) mM |