SP-B C-terminal (residues 59-80) peptide in DPC micelles
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 96.8 % (152 of 157) | 97.0 % (131 of 135) | 95.5 % (21 of 22) |
Backbone | 95.4 % (62 of 65) | 95.5 % (42 of 44) | 95.2 % (20 of 21) |
Sidechain | 97.8 % (90 of 92) | 97.8 % (89 of 91) | 100.0 % (1 of 1) |
Methyl | 100.0 % (21 of 21) | 100.0 % (21 of 21) |
1. entity
DTLLGRILPQ LVCRLVLRCS IDSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 315 K, pH 4.5 (±0.5), Details 1.2mM SP-B residues 59-80 in 80mM DPC-d38, pH 4.5, 50mM phosphate buffer, 5% D2O, reduced with 20mM TCEP, final sample volume 110uL
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 15N] | 1.2 mM | |
2 | D2O | [U-100% 2H] | 5 % | |
3 | DPC | [U-100% 2H] | 80 mM | |
4 | TSP | natural abundance | 1 mM | |
5 | sodium phosphate | natural abundance | 50 mM | |
6 | H2O | natural abundance | 95 % |