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TICAM-1 TIR domain structure
Authors
Enokizono, Y., Kumeta, H., Funami, K., Horiuchi, M., Sarmiento, J., Yamashita, K., Standley, D.M., Matsumoto, M., Seya, T., Inagaki, F.
Assembly
TICAM-1 TIR domain
Entity
1. TICAM-1 TIR domain (polymer), 168 monomers, 19102.43 Da Detail

MESSSEQKFY NFVILHARAD EHIALRVREK LEALGVPDGA TFCEDFQVHG RGELSCLQDA IDHSAFIILL LTSNFDCRLS LHQVNQAMMS NLTRQGSPDC VIPFLPLESS PAQLSSDTAS LLSGLVRLDE HSQIFARKVA NTFKPHRLQA RKAMWRKEQD LEHHHHHH


Formula weight
19102.43 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 97.3 %, Completeness (bb): 99.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All97.3 % (1907 of 1960)98.3 % (1001 of 1018)95.7 % (731 of 764)98.3 % (175 of 178)
Backbone99.3 % (989 of 996)99.4 % (334 of 336)99.4 % (495 of 498)98.8 % (160 of 162)
Sidechain95.9 % (1080 of 1126)97.8 % (667 of 682)93.0 % (398 of 428)93.8 % (15 of 16)
Aromatic73.5 % (119 of 162)84.0 % (68 of 81)62.5 % (50 of 80)100.0 % (1 of 1)
Methyl100.0 % (182 of 182)100.0 % (91 of 91)100.0 % (91 of 91)

1. TICAM1TIR

MESSSEQKFY NFVILHARAD EHIALRVREK LEALGVPDGA TFCEDFQVHG RGELSCLQDA IDHSAFIILL LTSNFDCRLS LHQVNQAMMS NLTRQGSPDC VIPFLPLESS PAQLSSDTAS LLSGLVRLDE HSQIFARKVA NTFKPHRLQA RKAMWRKEQD LEHHHHHH

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0, Details TICAM-1 TIR (387-545)


#NameIsotope labelingTypeConcentration
1TICAM-1 TIR[U-99% 13C; U-99% 15N]0.5 mM
2AcOH Buffernatural abundance20 mM
3DTTnatural abundance5 mM
4DSSnatural abundance0.02 mg/mL
5H20natural abundance90 %
6D20natural abundance10 %

LACS Plot; CA
Referencing offset: 0.06 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.06 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.02 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.21 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2M1X, Strand ID: A Detail


Release date
2012-12-20
Citation
Structures and interface mapping of the TIR domain-containing adaptor molecules involved in interferon signaling
Enokizono, Y., Kumeta, H., Funami, K., Horiuchi, M., Sarmiento, J., Yamashita, K., Standley, D.M., Matsumoto, M., Seya, T., Inagaki, F.
Proc. Natl. Acad. Sci. U. S. A. (2013), 110, 19908-19913, PubMed 24255114 , DOI 10.1073/pnas.1222811110 ,
Related entities 1. TICAM-1 TIR domain, : 1 : 13 entities Detail
Interaction partners 1. TICAM-1 TIR domain, : 13 interactors Detail
Experiments performed 17 experiments Detail
NMR combined restraints 4 contents Detail
Keywords Interferon, TICAM-1, TIR domain, TRIF, innate immunity