NMR structure of the glycosylated conotoxin CcTx from Conus consors
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 2:CYS13:SG | 2:CYS26:SG |
2 | disulfide | sing | 2:CYS12:SG | 2:CYS21:SG |
3 | disulfide | sing | 2:CYS24:SG | 2:CYS30:SG |
Polymer type: polysaccharide(D) polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 84.6 % (209 of 247) | 93.5 % (130 of 139) | 73.1 % (79 of 108) |
Backbone | 76.1 % (102 of 134) | 94.5 % (52 of 55) | 63.3 % (50 of 79) |
Sidechain | 94.9 % (131 of 138) | 92.9 % (78 of 84) | 98.1 % (53 of 54) |
Aromatic | 78.9 % (15 of 19) | 60.0 % (6 of 10) | 100.0 % (9 of 9) |
Methyl | 100.0 % (24 of 24) | 100.0 % (12 of 12) | 100.0 % (12 of 12) |
2. CONOTOXIN CCTX
AXWLVPSQIT TCCGYNXGTM CXSCMCTNTCSolvent system 95 % H2O/ 5% D2O, Pressure 1 atm, Temperature 292.000 K, pH 3.000, Details 1.1 mmol/l
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE | [U-13C; U-15N] | 1.1 mM | |
2 | CONOTOXIN_CCTX | [U-13C; U-15N] | 1.1 mM |
Polymer type: polysaccharide(D) polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 42.3 % (209 of 494) | 46.8 % (130 of 278) | 36.6 % (79 of 216) |
Backbone | 38.1 % (102 of 268) | 47.3 % (52 of 110) | 31.6 % (50 of 158) |
Sidechain | 47.5 % (131 of 276) | 46.4 % (78 of 168) | 49.1 % (53 of 108) |
Aromatic | 39.5 % (15 of 38) | 30.0 % (6 of 20) | 50.0 % (9 of 18) |
Methyl | 50.0 % (24 of 48) | 50.0 % (12 of 24) | 50.0 % (12 of 24) |
2. CONOTOXIN CCTX
AXWLVPSQIT TCCGYNXGTM CXSCMCTNTCSolvent system 95 % H2O/ 5% D2O, Pressure 1 atm, Temperature 292.000 K, pH 3.000, Details 1.1 mmol/l
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE | [U-13C; U-15N] | 1.1 mM | |
2 | CONOTOXIN_CCTX | [U-13C; U-15N] | 1.1 mM |