BMRBj - BMREntryMaster

Found 1 Document (0.809 seconds)

BMRB: 18910

3ZGK

NMR solution structure of the AVR3a11 from Phytophthora Capsici

Authors

Tolchard, J.L., Chambers, V.S., Boutemy, L.S., Gathercole, R.L., Banfield, M.J., Blumenschein, T.M.

Assembly

NMR solution structure of the AVR3a11 from Phytophthora Capsici

Entity

1. NMR solution structure of the AVR3a11 from Phytophthora Capsici (polymer, Thiol state: not present), 72 monomers, 8253.355 Da Detail

GPGLTDLFKT EKAAVKKMAK AIMADPSKAD DVYQKWADKG YTLTQLSDFL KSKTRGKYDR VYNGYMTYRD YV

Formula weight

8253.355 Da

Source organism

Phytophthora capsici

Exptl. method

Refine. method

DISTANCE GEOMETRY, SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 71.0 %, Completeness (bb): 70.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.0 % (612 of 862)	73.8 % (332 of 450)	64.5 % (218 of 338)	83.8 % (62 of 74)
Backbone	70.1 % (300 of 428)	78.2 % (115 of 147)	60.2 % (127 of 211)	82.9 % (58 of 70)
Sidechain	74.1 % (371 of 501)	71.6 % (217 of 303)	77.3 % (150 of 194)	100.0 % (4 of 4)
Aromatic	81.8 % (72 of 88)	81.8 % (36 of 44)	81.4 % (35 of 43)	100.0 % (1 of 1)
Methyl	75.8 % (50 of 66)	81.8 % (27 of 33)	69.7 % (23 of 33)

1. AVR3A11

GPGLTDLFKT EKAAVKKMAK AIMADPSKAD DVYQKWADKG YTLTQLSDFL KSKTRGKYDR VYNGYMTYRD YV

Show sample condition

Sample

Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 3ZGK, Strand ID: A Detail

Release date

2016-08-02

Citation

NMR SOLUTION STRUCTURE OF THE AVR3A11 FROM PHYTOPHTHORA CAPSI
Tolchard, J.L., Chambers, V.S., Boutemy, L.S., Gathercole, R.L., Banfield, M.J., Blumenschein, T.M.
Not known

Related entities 1. NMR solution structure of the AVR3a11 from Phytophthora Capsici, : 1 : 1 : 17 entities Detail

Experiments performed 11 experiments Detail

1 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

2 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

3 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

4 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

5 CBCACONH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

6 HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

7 CCCONH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

8 H(CCO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

9 1H-15N TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

10 Aromatic 1H-13C TROSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

11 hbCBcgcdceHE

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 10% water/90% D2O, 200 uM DSS, 0.02% NaN3, Pressure 1.000 atm, Temperature 298.000 K, pH 8.800, Details AVR3a11 1mM

#	Name	Isotope labeling	Type	Concentration
1	AVR3A11	[U-13C; U-15N]		1 mM

nullKeywords AVR3a11, Phytophthora Capsici, Plant Diseases, RXLR, UNKNOWN FUNCTION