BMRBj - BMREntryMaster

Found 1 Document (0.541 seconds)

BMRB: 18922

8JO2

1H, 13C and 15N assignments of the dimeric receiver domain of PmrAn response regulator from Klebsiella pneumonia

Authors

Luo, S.

Assembly

PmrAn dimer

Entity

1. PmrAn dimer (polymer, Thiol state: all free), 123 monomers, 13670.54 × 2 Da Detail

MKILVIEDDA LLLQGLILAM QSEGYVCDGV STAHEAALSL ASNHYSLIVL DLGLPDEDGL HFLSRMRREK MTQPVLILTA RDTLEDRISG LDTGADDYLV KPFALEELNA RIRALLRRHN NQG

Total weight

27341.08 Da

Max. entity weight

13670.54 Da

Source organism

Klebsiella pneumoniae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 65.7 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.7 % (921 of 1402)	61.1 % (442 of 723)	65.5 % (361 of 551)	92.2 % (118 of 128)
Backbone	97.1 % (711 of 732)	96.0 % (241 of 251)	97.5 % (352 of 361)	98.3 % (118 of 120)
Sidechain	41.4 % (325 of 785)	42.6 % (201 of 472)	40.7 % (124 of 305)	0.0 % (0 of 8)
Aromatic	6.7 % (4 of 60)	13.3 % (4 of 30)	0.0 % (0 of 30)
Methyl	18.2 % (32 of 176)	19.3 % (17 of 88)	17.0 % (15 of 88)

1. PmrAn

MKILVIEDDA LLLQGLILAM QSEGYVCDGV STAHEAALSL ASNHYSLIVL DLGLPDEDGL HFLSRMRREK MTQPVLILTA RDTLEDRISG LDTGADDYLV KPFALEELNA RIRALLRRHN NQG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 8

#	Name	Isotope labeling	Type	Concentration
1	PmrAn	[U-100% 13C; U-100% 15N; U-80% 2H]		0.5 ~ 1.0 mM

Release date

2014-04-21

Citation

Structural basis of a physical blockage mechanism for the interaction of response regulator PmrA with connector protein PmrD from Klebsiella pneumoniae
Luo, S., Lou, Y., Rajasekaran, M., Chang, Y., Hsiao, C., Chen, C.
J. Biol. Chem. (2013), 288, 25551-25561, PubMed 23861396 , DOI 10.1074/jbc.M113.481978 , Abstract

Related entities 1. PmrAn dimer, : 1 : 3 : 292 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr18922_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr18922_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	897		100.0 (chain: 1, length: 123)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 123, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 352
  - ¹H chemical shifts: 428
  - ¹⁵N chemical shifts: 117
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	551	352	63.9
¹H chemical shifts	723	408	56.4
¹⁵N chemical shifts	137	117	85.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	246	238	96.7
¹H chemical shifts	251	237	94.4
¹⁵N chemical shifts	120	117	97.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	305	114	37.4
¹H chemical shifts	472	171	36.2
¹⁵N chemical shifts	17	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	92	11	12.0
¹H chemical shifts	92	10	10.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	30	0	0.0
¹H chemical shifts	30	0	0.0

Keywords PmrA, receiver domain, response regulator

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.541 seconds)

BMRB: 18922

Sample

Related entities 1. PmrAn dimer, : 1 : 3 : 292 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail