1H, 13C and 15N assignments of the dimeric receiver domain of PmrAn response regulator from Klebsiella pneumonia
MKILVIEDDA LLLQGLILAM QSEGYVCDGV STAHEAALSL ASNHYSLIVL DLGLPDEDGL HFLSRMRREK MTQPVLILTA RDTLEDRISG LDTGADDYLV KPFALEELNA RIRALLRRHN NQG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 65.7 % (921 of 1402) | 61.1 % (442 of 723) | 65.5 % (361 of 551) | 92.2 % (118 of 128) |
Backbone | 97.1 % (711 of 732) | 96.0 % (241 of 251) | 97.5 % (352 of 361) | 98.3 % (118 of 120) |
Sidechain | 41.4 % (325 of 785) | 42.6 % (201 of 472) | 40.7 % (124 of 305) | 0.0 % (0 of 8) |
Aromatic | 6.7 % (4 of 60) | 13.3 % (4 of 30) | 0.0 % (0 of 30) | |
Methyl | 18.2 % (32 of 176) | 19.3 % (17 of 88) | 17.0 % (15 of 88) |
1. PmrAn
MKILVIEDDA LLLQGLILAM QSEGYVCDGV STAHEAALSL ASNHYSLIVL DLGLPDEDGL HFLSRMRREK MTQPVLILTA RDTLEDRISG LDTGADDYLV KPFALEELNA RIRALLRRHN NQGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PmrAn | [U-100% 13C; U-100% 15N; U-80% 2H] | 0.5 ~ 1.0 mM |