Solution structure of MCh-1: A novel inhibitor cystine knot peptide from Momordica charantia
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS2:SG | 1:CYS19:SG |
2 | disulfide | sing | 1:CYS7:SG | 1:CYS21:SG |
3 | disulfide | sing | 1:CYS15:SG | 1:CYS31:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 93.5 % (144 of 154) | 93.5 % (144 of 154) |
Backbone | 98.5 % (67 of 68) | 98.5 % (67 of 68) |
Sidechain | 89.5 % (77 of 86) | 89.5 % (77 of 86) |
Aromatic | 40.0 % (2 of 5) | 40.0 % (2 of 5) |
Methyl | 89.5 % (17 of 19) | 89.5 % (17 of 19) |
1. MCh-1
GCAGKSCNIL GSDPCDAGCF CLPVGIVAGV CVSolvent system 90% H2O/10% D2O, Temperature 298 (±0.2) K, pH 5 (±0.2), Details Lyophilized samples was dissolved in 90%H2O/10% D2O with an additional of approximately 27% acetonitrile
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MCh-1 | natural abundance | 3 (±0.2) mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % | |
4 | acetonitrile | natural abundance | 27 % |