BMRB: 18981

DNA containing a cluster of 8-oxo-guanine and THF lesion

Authors

Zalesak, J., Jourdan, M., Constant, J., Lourdin, M.

Assembly

DNA duplex containing a cluster of mutagenic 8-oxo-guanine and abasic site lesion

Entity

1. DNA A strand (polymer, Thiol state: not present), 11 monomers, 3209.012 Da Detail

CGCTCXCACG C

2. DNA B strand (polymer, Thiol state: not present), 11 monomers, 3534.218 Da Detail

GCGTGGGAXC G

Total weight

6743.23 Da

Max. entity weight

3534.218 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 86.2 %, Completeness (bb): 82.5 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	86.2 % (188 of 218)	85.4 % (169 of 198)	95.0 % (19 of 20)
Suger, PO4	82.5 % (132 of 160)	80.7 % (113 of 140)	95.0 % (19 of 20)
Nucleobase	96.6 % (56 of 58)	96.6 % (56 of 58)
Aromatic	95.0 % (38 of 40)	95.0 % (38 of 40)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. DNA A strand

CGCTCXCACG C

2. DNA B strand

GCGTGGGAXC G

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	DNA_A_strand	natural abundance		0.97 mM
2	DNA_B_strand	natural abundance		0.97 mM
3	D2O	natural abundance		100 %

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Release date

2014-01-05

Citation

Structure and dynamics of DNA duplexes containing a cluster of mutagenic 8-oxoguanine and abasic site lesions
Zaleak, J., Lourdin, M., Krej, L., Constant, J., Jourdan, M.
J. Mol. Biol. (2014), 426, 1524-1538, PubMed 24384094 , DOI 10.1016/j.jmb.2013.12.022 , Abstract

Experiments performed 3 experiments Detail