Search

protein structure
Authors
Noh, Y.J., Mercier, P., Spratt, D.E., Shaw, G.S.
Assembly
protein
Entity
1. protein, entity 1 (polymer), 141 monomers, 15445.44 Da Detail

EEYVLQAGGV LCPQPGCGMG LLVEPDCRKV TCQNGCGYVF CRNCLQGYHI GECLPEGTGA SATNSCEYTV DPNRAAEARW DEASNVTIKV STKPCPKCRT PTERDGGCMH MVCTRAGCGF EWCWVCQTEW TRDCMGAHWF G


2. protein, entity ZN (non-polymer, Thiol state: not present), 65.409 × 4 Da
Total weight
15707.076 Da
Max. entity weight
15445.44 Da
Source organism
Drosophila melanogaster
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.3 %, Completeness: 89.1 %, Completeness (bb): 94.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All89.1 % (1363 of 1530)86.5 % (684 of 791)91.9 % (543 of 591)91.9 % (136 of 148)
Backbone94.6 % (785 of 830)93.5 % (272 of 291)95.8 % (389 of 406)93.2 % (124 of 133)
Sidechain84.7 % (698 of 824)82.4 % (412 of 500)88.7 % (274 of 309)80.0 % (12 of 15)
Aromatic82.1 % (110 of 134)83.6 % (56 of 67)79.0 % (49 of 62)100.0 % (5 of 5)
Methyl85.7 % (96 of 112)85.7 % (48 of 56)85.7 % (48 of 56)

1. entity 1

EEYVLQAGGV LCPQPGCGMG LLVEPDCRKV TCQNGCGYVF CRNCLQGYHI GECLPEGTGA SATNSCEYTV DPNRAAEARW DEASNVTIKV STKPCPKCRT PTERDGGCMH MVCTRAGCGF EWCWVCQTEW TRDCMGAHWF G

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details Added Roche EDTA-free protease inhibitor cocktail (~10 uM) to limit proteolysis during data collection


#NameIsotope labelingTypeConcentration
1TRISnatural abundance25 mM
2sodium chloridenatural abundance150 mM
3DTTnatural abundance5 mM
4entity_1[U-98% 15N]500 uM
5H20natural abundance90 %
6D20natural abundance10 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details Added Roche EDTA-free protease inhibitor cocktail (~10 uM) to limit proteolysis during data collection


#NameIsotope labelingTypeConcentration
7TRISnatural abundance25 mM
8sodium chloridenatural abundance150 mM
9DTTnatural abundance5 mM
10entity_1[U-98% 13C; U-98% 15N]500 uM
11H2Onatural abundance90 %
12D2Onatural abundance10 %
Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details Added Roche EDTA-free protease inhibitor cocktail (~10 uM) to limit proteolysis during data collection


#NameIsotope labelingTypeConcentration
13TRISnatural abundance25 mM
14sodium chloridenatural abundance150 mM
15DTTnatural abundance5 mM
16entity_1[U-98% 13C; U-98% 15N]500 uM
17H2Onatural abundance90 %
18D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.06 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.02 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.38 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2M48, Strand ID: A Detail


Release date
2012-01-23
Citation
A molecular explanation for the recessive nature of parkin-linked Parkinson's disease
Spratt, D.E., Martinez-Torres, R., Noh, Y.J., Mercier, P., Manczyk, N., Barber, K.R., Aguirre, J.D., Burchell, L., Purkiss, A., Walden, H., Shaw, G.S.
Nat. Commun. (2013), 4, 1983-1983, PubMed 23770917 , DOI 10.1038/ncomms2983 ,
Related entities 1. protein, entity 1, : 1 : 1 : 140 entities Detail
Experiments performed 20 experiments Detail
NMR combined restraints 4 contents Detail
Keywords E3 ligase, IBR, Parkin, RING, Zn-binding