NMR structure of the complex of an N-terminally acetylated alpha-synuclein peptide with calmodulin
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 47.6 % (894 of 1879) | 39.9 % (389 of 974) | 47.7 % (347 of 728) | 89.3 % (158 of 177) |
Backbone | 83.0 % (828 of 998) | 98.8 % (341 of 345) | 67.6 % (330 of 488) | 95.2 % (157 of 165) |
Sidechain | 20.8 % (215 of 1035) | 7.6 % (48 of 629) | 42.1 % (166 of 394) | 8.3 % (1 of 12) |
Aromatic | 0.0 % (0 of 110) | 0.0 % (0 of 55) | 0.0 % (0 of 55) | |
Methyl | 18.7 % (31 of 166) | 8.4 % (7 of 83) | 28.9 % (24 of 83) |
1. calmodulin
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK2. peptide
MDVFMKGLSK AKEGVVAAAXSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | calmodulin | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | peptide | natural abundance | 600 uM | |
3 | MES | natural abundance | 50 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | CALCIUM ION | natural abundance | 3 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | peptide | [U-13C; U-15N]-Met,Val,Phe,Gly,Leu,Ala | 700 uM | |
9 | calmodulin | natural abundance | 700 uM | |
10 | MES | natural abundance | 50 mM | |
11 | potassium chloride | natural abundance | 100 mM | |
12 | CALCIUM ION | natural abundance | 3 mM | |
13 | sodium azide | natural abundance | .1 % | |
14 | H2O | natural abundance | 95 % | |
15 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | calmodulin | [U-99% 13C; U-99% 15N] | 150 uM | |
17 | peptide | natural abundance | 150 uM | |
18 | MES | natural abundance | 50 mM | |
19 | potassium chloride | natural abundance | 100 mM | |
20 | CALCIUM ION | natural abundance | 3 mM | |
21 | Pf1 phage | natural abundance | 10 mg/mL | |
22 | H2O | natural abundance | 95 % | |
23 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | calmodulin | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | peptide | natural abundance | 600 uM | |
3 | MES | natural abundance | 50 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | CALCIUM ION | natural abundance | 3 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | peptide | [U-13C; U-15N]-Met,Val,Phe,Gly,Leu,Ala | 700 uM | |
9 | calmodulin | natural abundance | 700 uM | |
10 | MES | natural abundance | 50 mM | |
11 | potassium chloride | natural abundance | 100 mM | |
12 | CALCIUM ION | natural abundance | 3 mM | |
13 | sodium azide | natural abundance | .1 % | |
14 | H2O | natural abundance | 95 % | |
15 | D2O | natural abundance | 5 % |
Bruker Avance - 900 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | calmodulin | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | peptide | natural abundance | 600 uM | |
3 | MES | natural abundance | 50 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | CALCIUM ION | natural abundance | 3 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | calmodulin | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | peptide | natural abundance | 600 uM | |
3 | MES | natural abundance | 50 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | CALCIUM ION | natural abundance | 3 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Bruker Avance - 900 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | peptide | [U-13C; U-15N]-Met,Val,Phe,Gly,Leu,Ala | 700 uM | |
9 | calmodulin | natural abundance | 700 uM | |
10 | MES | natural abundance | 50 mM | |
11 | potassium chloride | natural abundance | 100 mM | |
12 | CALCIUM ION | natural abundance | 3 mM | |
13 | sodium azide | natural abundance | .1 % | |
14 | H2O | natural abundance | 95 % | |
15 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | peptide | [U-13C; U-15N]-Met,Val,Phe,Gly,Leu,Ala | 700 uM | |
9 | calmodulin | natural abundance | 700 uM | |
10 | MES | natural abundance | 50 mM | |
11 | potassium chloride | natural abundance | 100 mM | |
12 | CALCIUM ION | natural abundance | 3 mM | |
13 | sodium azide | natural abundance | .1 % | |
14 | H2O | natural abundance | 95 % | |
15 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | calmodulin | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | peptide | natural abundance | 600 uM | |
3 | MES | natural abundance | 50 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | CALCIUM ION | natural abundance | 3 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | peptide | [U-13C; U-15N]-Met,Val,Phe,Gly,Leu,Ala | 700 uM | |
9 | calmodulin | natural abundance | 700 uM | |
10 | MES | natural abundance | 50 mM | |
11 | potassium chloride | natural abundance | 100 mM | |
12 | CALCIUM ION | natural abundance | 3 mM | |
13 | sodium azide | natural abundance | .1 % | |
14 | H2O | natural abundance | 95 % | |
15 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | calmodulin | [U-99% 13C; U-99% 15N] | 150 uM | |
17 | peptide | natural abundance | 150 uM | |
18 | MES | natural abundance | 50 mM | |
19 | potassium chloride | natural abundance | 100 mM | |
20 | CALCIUM ION | natural abundance | 3 mM | |
21 | Pf1 phage | natural abundance | 10 mg/mL | |
22 | H2O | natural abundance | 95 % | |
23 | D2O | natural abundance | 5 % |
Bruker Avance - 900 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | calmodulin | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | peptide | natural abundance | 600 uM | |
3 | MES | natural abundance | 50 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | CALCIUM ION | natural abundance | 3 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | calmodulin | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | peptide | natural abundance | 600 uM | |
3 | MES | natural abundance | 50 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | CALCIUM ION | natural abundance | 3 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Bruker Avance - 900 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | peptide | [U-13C; U-15N]-Met,Val,Phe,Gly,Leu,Ala | 700 uM | |
9 | calmodulin | natural abundance | 700 uM | |
10 | MES | natural abundance | 50 mM | |
11 | potassium chloride | natural abundance | 100 mM | |
12 | CALCIUM ION | natural abundance | 3 mM | |
13 | sodium azide | natural abundance | .1 % | |
14 | H2O | natural abundance | 95 % | |
15 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | peptide | [U-13C; U-15N]-Met,Val,Phe,Gly,Leu,Ala | 700 uM | |
9 | calmodulin | natural abundance | 700 uM | |
10 | MES | natural abundance | 50 mM | |
11 | potassium chloride | natural abundance | 100 mM | |
12 | CALCIUM ION | natural abundance | 3 mM | |
13 | sodium azide | natural abundance | .1 % | |
14 | H2O | natural abundance | 95 % | |
15 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz
State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | calmodulin | [U-99% 13C; U-99% 15N] | 150 uM | |
17 | peptide | natural abundance | 150 uM | |
18 | MES | natural abundance | 50 mM | |
19 | potassium chloride | natural abundance | 100 mM | |
20 | CALCIUM ION | natural abundance | 3 mM | |
21 | Pf1 phage | natural abundance | 10 mg/mL | |
22 | H2O | natural abundance | 95 % | |
23 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.36
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | calmodulin | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | peptide | natural abundance | 600 uM | |
3 | MES | natural abundance | 50 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | CALCIUM ION | natural abundance | 3 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints | combined_19036_2m55.nef |
Input source #2: Coordindates | 2m55.cif |
Diamagnetism of the molecular assembly | False (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | True (see coodinates for details) |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
Ptnr_site_1 | Ptnr_site_2 | Redox_state_prediction_1 | Redox_state_prediction_2 | Distance (Ã…) |
---|---|---|---|---|
2:1:ACE:C | 2:2:MET:N | unknown | unknown | n/a |
2:20:ALA:C | 2:21:NH2:N | unknown | unknown | n/a |
1:129:ASP:OD1 | 3:4:CA:CA | unknown | unknown | n/a |
1:26:THR:O | 3:1:CA:CA | unknown | unknown | n/a |
1:31:GLU:OE2 | 3:1:CA:CA | unknown | unknown | n/a |
1:58:ASP:OD2 | 3:2:CA:CA | unknown | unknown | n/a |
1:140:GLU:OE1 | 3:4:CA:CA | unknown | unknown | n/a |
1:62:THR:O | 3:2:CA:CA | unknown | unknown | n/a |
1:131:ASP:OD1 | 3:4:CA:CA | unknown | unknown | n/a |
1:93:ASP:OD1 | 3:3:CA:CA | unknown | unknown | n/a |
1:60:ASN:OD1 | 3:2:CA:CA | unknown | unknown | n/a |
1:56:ASP:OD1 | 3:2:CA:CA | unknown | unknown | n/a |
1:135:GLN:O | 3:4:CA:CA | unknown | unknown | n/a |
1:67:GLU:OE2 | 3:2:CA:CA | unknown | unknown | n/a |
1:97:ASN:OD1 | 3:3:CA:CA | unknown | unknown | n/a |
1:99:TYR:O | 3:3:CA:CA | unknown | unknown | n/a |
1:24:ASP:OD2 | 3:1:CA:CA | unknown | unknown | n/a |
1:22:ASP:OD2 | 3:1:CA:CA | unknown | unknown | n/a |
1:20:ASP:OD1 | 3:1:CA:CA | unknown | unknown | n/a |
1:95:ASP:OD2 | 3:3:CA:CA | unknown | unknown | n/a |
1:104:GLU:OE2 | 3:3:CA:CA | unknown | unknown | n/a |
1:140:GLU:OE2 | 3:4:CA:CA | unknown | unknown | n/a |
1:31:GLU:OE1 | 3:1:CA:CA | unknown | unknown | n/a |
1:58:ASP:OD1 | 3:2:CA:CA | unknown | unknown | n/a |
1:133:ASP:OD1 | 3:4:CA:CA | unknown | unknown | n/a |
1:67:GLU:OE1 | 3:2:CA:CA | unknown | unknown | n/a |
1:104:GLU:OE1 | 3:3:CA:CA | unknown | unknown | n/a |
1:24:ASP:OD1 | 3:1:CA:CA | unknown | unknown | n/a |
Non-standard residues
Chain_ID | Seq_ID | Comp_ID | Chem_comp_name | Experimental evidences |
---|---|---|---|---|
B | 200 | ACE | ACETYL GROUP | Coordinates |
B | 220 | NH2 | AMINO GROUP | Coordinates |
C | 1 | CA | CALCIUM ION | None |
C | 2 | CA | CALCIUM ION | None |
C | 3 | CA | CALCIUM ION | None |
C | 4 | CA | CALCIUM ION | None |
Sequence alignments
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||||||||||||||||||||||||||||||||||||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
200-----210-------220 XMDVFMKGLSKAKEGVVAAAX ||||||||||||||||||||| XMDVFMKGLSKAKEGVVAAAX --------10--------20-
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 148 | 0 | 0 | 100.0 |
B | B | 21 | 0 | 0 | 100.0 |
Content subtype: combined_19036_2m55.nef
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||||||||||||||||||||||||||||||||||||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
200-----210-------220 XMDVFMKGLSKAKEGVVAAAX |||||||| |||||||||| .MDVFMKGL.KAKEGVVAAA 200-----210---------
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 867 | 303 | 34.9 |
13C chemical shifts | 645 | 284 | 44.0 |
15N chemical shifts | 164 | 144 | 87.8 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 305 | 303 | 99.3 |
13C chemical shifts | 296 | 147 | 49.7 |
15N chemical shifts | 146 | 144 | 98.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 562 | 0 | 0.0 |
13C chemical shifts | 349 | 137 | 39.3 |
15N chemical shifts | 18 | 0 | 0.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 80 | 0 | 0.0 |
13C chemical shifts | 80 | 11 | 13.8 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 50 | 0 | 0.0 |
13C chemical shifts | 50 | 0 | 0.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 111 | 38 | 34.2 |
13C chemical shifts | 85 | 24 | 28.2 |
15N chemical shifts | 20 | 13 | 65.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 41 | 38 | 92.7 |
13C chemical shifts | 38 | 13 | 34.2 |
15N chemical shifts | 20 | 13 | 65.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 70 | 0 | 0.0 |
13C chemical shifts | 47 | 11 | 23.4 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 15 | 0 | 0.0 |
13C chemical shifts | 15 | 4 | 26.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 5 | 0 | 0.0 |
13C chemical shifts | 5 | 0 | 0.0 |
Covalent bonds
Distance restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||||||||||||||||||||||||||||||||||||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
200-----210-------220 XMDVFMKGLSKAKEGVVAAAX ||||||||||||||||||| .MDVFMKGLSKAKEGVVAAA 200-----210---------
Dihedral angle restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||| ||| ADQLTEEQIAEFKEAFSLFDKDG.GTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMK..DSEEEIREAFRVFDKDG.GYI -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||||||||||||||||||||||||||||||||||||||||||||||| SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
200-----210-------220 XMDVFMKGLSKAKEGVVAAAX |||||||||||||||||| ..DVFMKGLSKAKEGVVAAA 200-----210---------
RDC restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYI ||||||||||| ||||||||||||||||||||||| ||| |||| ||| |||||| ||||| ||| | ||| || || | || | ||| ...LTEEQIAEFKE.FSLFDKDGDGTITTKELGTVMRS.GQN.TEAE..DMI........GTIDFP.FLTMM.RKM..T.SEE.IR.AF.V.DK.G.GYI --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------- SAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK |||| |||||||||||||||||||| ||||| |||||||||| ||| SAAE.RHVMTNLGEKLTDEEVDEMI.EADID.DGQVNYEEFV..MTA -------110-------120-------130-------140-------