Backbone 1H, 15N Chemical Shift Assignments for wild-type Escherichia coli Adenylate Kinase with 20mM ADP and Magnesium at 298K
MRIILLGAPG AGKGTQAQFI MEKYGIPQIS TGDMLRAAVK SGSELGKQAK DIMDAGKLVT DELVIALVKE RIAQEDCRNG FLLDGFPRTI PQADAMKEAG INVDYVLEFD VPDELIVDRI VGRRVHAPSG RVYHVKFNPP KVEGKDDVTG EELTTRKDDQ EETVRKRLVE YHQMTAPLIG YYSKEAEAGN TKYAKVDGTK PVAEVRADLE KILG
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 25.2 % (378 of 1498) | 15.8 % (202 of 1282) | 81.5 % (176 of 216) |
Backbone | 56.2 % (361 of 642) | 42.2 % (185 of 438) | 86.3 % (176 of 204) |
Sidechain | 2.0 % (17 of 856) | 2.0 % (17 of 844) | 0.0 % (0 of 12) |
Aromatic | 0.0 % (0 of 59) | 0.0 % (0 of 59) | |
Methyl | 4.7 % (6 of 128) | 4.7 % (6 of 128) |
1. adenylate kinase wild type
MRIILLGAPG AGKGTQAQFI MEKYGIPQIS TGDMLRAAVK SGSELGKQAK DIMDAGKLVT DELVIALVKE RIAQEDCRNG FLLDGFPRTI PQADAMKEAG INVDYVLEFD VPDELIVDRI VGRRVHAPSG RVYHVKFNPP KVEGKDDVTG EELTTRKDDQ EETVRKRLVE YHQMTAPLIG YYSKEAEAGN TKYAKVDGTK PVAEVRADLE KILGSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | adenylate kinase | [U-13C; U-15N] | 2 mM | |
2 | ADP | natural abundance | 20 mM | |
3 | MG | natural abundance | 20 mM | |
4 | D2O | natural abundance | 5 % | |
5 | H2O | natural abundance | 95 % |