BMRBj - BMREntryMaster

Found 1 Document (0.46 seconds)

BMRB: 19114

4E-BP2

Authors

Forman-Kay, J., Bah, A.

Assembly

4E-BP2

Entity

1. 4E-BP2 (polymer, Thiol state: all free), 128 monomers, 13800.20 × 2 Da Detail

MPLGSPEFMS SSAGSGHQPS QSRAIPTRTV AISDAAQLPH DYCTTPGGTL FSTTPGGTRI IYDRKFLLDR RNSPMAQTPP CHLPNIPGVT SPGTLIEDSK VEVNNLDNLD NHDRKHAVGD DAQFEMDI

Total weight

27600.4 Da

Max. entity weight

13800.2 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 57.3 %, Completeness (bb): 93.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	57.3 % (803 of 1402)	34.3 % (250 of 729)	79.9 % (437 of 547)	92.1 % (116 of 126)
Backbone	93.0 % (690 of 742)	84.2 % (213 of 253)	97.1 % (363 of 374)	99.1 % (114 of 115)
Sidechain	29.4 % (229 of 778)	7.8 % (37 of 476)	65.3 % (190 of 291)	18.2 % (2 of 11)
Aromatic	0.0 % (0 of 76)	0.0 % (0 of 38)	0.0 % (0 of 38)
Methyl	19.7 % (24 of 122)	4.9 % (3 of 61)	34.4 % (21 of 61)

1. 4E-BP2

MPLGSPEFMS SSAGSGHQPS QSRAIPTRTV AISDAAQLPH DYCTTPGGTL FSTTPGGTRI IYDRKFLLDR RNSPMAQTPP CHLPNIPGVT SPGTLIEDSK VEVNNLDNLD NHDRKHAVGD DAQFEMDI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	4E-BP2	[U-99% 13C; U-99% 15N]	800.0 ~ 1200.0 uM
2	DTT	natural abundance	2 mM
3	sodium chloride	natural abundance	100 mM
4	sodium phosphate	natural abundance	30 mM
5	EDTA	natural abundance	1 mM
6	benzamidine	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	-1078.012 Hz	internal	indirect	0.2514495
¹H	DSS	protons	-4287.19 Hz	internal	direct	1.0
¹⁵N	urea	nitrogen	-434.4172 Hz	internal	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	-1078.012 Hz	internal	indirect	0.2514495
¹H	DSS	protons	-4287.19 Hz	internal	direct	1.0
¹⁵N	urea	nitrogen	-434.4172 Hz	internal	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	-1078.012 Hz	internal	indirect	0.2514495
¹H	DSS	protons	-4287.19 Hz	internal	direct	1.0
¹⁵N	urea	nitrogen	-434.4172 Hz	internal	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	-1078.012 Hz	internal	indirect	0.2514495
¹H	DSS	protons	-4287.19 Hz	internal	direct	1.0
¹⁵N	urea	nitrogen	-434.4172 Hz	internal	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 1 Detail

Release date

2014-02-11

Citation 1

Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface
Lukhele, S., Bah, A., Lin, H., Sonenberg, N., Forman-Kay, J.D.
Structure (2013), 21, 2186-2196, PubMed 24207126 , DOI 10.1016/j.str.2013.08.030 , Abstract

Show more 1 citaion

Citation 2

Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch
Bah, A., Vernon, R., Siddiqui, Z., Krzeminski, M., Muhandiram, R., Zhao, C., Sonenberg, N., Kay, L., Forman-Kay, J.
Nature (2015), 519, 106-109, PubMed 25533957 , DOI 10.1038/nature13999 , Abstract

Hide non-primary 1 citaion

Related entities 1. 4E-BP2, : 20 entities Detail

Interaction partners 1. 4E-BP2, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr19114_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr19114_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	760		99.2 (chain: 1, length: 128)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 128, Sequence coverage: 99.2%
- Total number of assignments
  - ¹³C chemical shifts: 438
  - ¹H chemical shifts: 209
  - ¹⁵N chemical shifts: 113
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	547	438	80.1
¹H chemical shifts	729	209	28.7
¹⁵N chemical shifts	133	113	85.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	256	249	97.3
¹H chemical shifts	253	209	82.6
¹⁵N chemical shifts	115	113	98.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	291	189	64.9
¹H chemical shifts	476	0	0.0
¹⁵N chemical shifts	18	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	65	20	30.8
¹H chemical shifts	65	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	38	0	0.0
¹H chemical shifts	38	0	0.0

Keywords Fuzzy Complexes, Intrinsically Disordered Protein, NMR, folding, IDPs, Post translational modification

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Found 1 Document (0.46 seconds)

BMRB: 19114

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. 4E-BP2, : 20 entities Detail

Interaction partners 1. 4E-BP2, : 3 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail