BMRB: 19148

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase

Authors

Liu, B., Tabib-Salazar1, A., Doughty, P., Lewis, R., Ghosh, S., Parsy, M., Simpson, P., O'Dwyer, K., Matthews, S., Paget, M.

Assembly

actinobacterial transcription factor RbpA

Entity

1. actinobacterial transcription factor RbpA (polymer), 48 monomers, 5351.966 Da Detail

QAVEYACEKG HRFEMPFSVE AEIPPEWECK VCGAQALLVD GDGPEEKK

Formula weight

5351.966 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 86.1 %, Completeness (bb): 97.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.1 % (472 of 548)	83.0 % (239 of 288)	88.7 % (189 of 213)	93.6 % (44 of 47)
Backbone	97.9 % (274 of 280)	96.9 % (93 of 96)	97.9 % (137 of 140)	100.0 % (44 of 44)
Sidechain	77.2 % (241 of 312)	76.0 % (146 of 192)	81.2 % (95 of 117)	0.0 % (0 of 3)
Aromatic	59.1 % (26 of 44)	59.1 % (13 of 22)	61.9 % (13 of 21)	0.0 % (0 of 1)
Methyl	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (19 of 19)

1. entity

QAVEYACEKG HRFEMPFSVE AEIPPEWECK VCGAQALLVD GDGPEEKK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	beta-mercaptoethanol	natural abundance		3 mM
2	sodium chloride	natural abundance		500 mM
3	TRIS	natural abundance		20 mM
4	RbpA	natural abundance		0.0 ~ 0.0 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	glucose	carbon	0.0 ppm	internal	indirect	1.0
1H	water	protons	0.0 ppm	internal	indirect	1.0
15N	[15N] ammonium chloride	nitrogen	0.0 ppm	internal	indirect	1.0

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	glucose	carbon	0.0 ppm	internal	indirect	1.0
1H	water	protons	0.0 ppm	internal	indirect	1.0
15N	[15N] ammonium chloride	nitrogen	0.0 ppm	internal	indirect	1.0

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	glucose	carbon	0.0 ppm	internal	indirect	1.0
1H	water	protons	0.0 ppm	internal	indirect	1.0
15N	[15N] ammonium chloride	nitrogen	0.0 ppm	internal	indirect	1.0

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.48 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	glucose	carbon	0.0 ppm	internal	indirect	1.0
1H	water	protons	0.0 ppm	internal	indirect	1.0
15N	[15N] ammonium chloride	nitrogen	0.0 ppm	internal	indirect	1.0

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 10 models in PDB: 2M6O, Strand ID: A Detail

---

Release date

2013-05-05

Citation

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase
Tabib-Salazar1, A., Liu, B., Doughty, P., Lewis, R., Ghosh, S., Parsy, M., Simpson, P., O'Dwyer, K., Matthews, S., Paget, M.
Nucleic Acids Res. (2013), 41, 5679-5691, PubMed 23605043 , DOI 10.1093/nar/gkt277 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 7 entries Detail

Related entities 1. actinobacterial transcription factor RbpA, : 1 : 1 : 34 entities Detail

Experiments performed 9 experiments Detail