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The solution structure of the J-domain of human DnaJA1
Authors
Stark, J.L., Mehla, K., Chaika, N., Acton, T.B., Xiao, R., Singh, P.K., Montelione, G.T., Powers, R.
Assembly
J-domain of human DnaJA1
Entity
1. J-domain of human DnaJA1 (polymer, Thiol state: not present), 77 monomers, 9073.118 Da Detail

MGHHHHHHSH MVKETTYYDV LGVKPNATQE ELKKAYRKLA LKYHPDKNPN EGEKFKQISQ AYEVLSDAKK RELYDKG


Formula weight
9073.118 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
distance geometry, torsion angle dynamics, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 88.3 %, Completeness: 75.2 %, Completeness (bb): 86.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All75.2 % (708 of 942)73.9 % (368 of 498)76.1 % (277 of 364)78.8 % (63 of 80)
Backbone86.0 % (392 of 456)85.2 % (132 of 155)86.8 % (197 of 227)85.1 % (63 of 74)
Sidechain67.8 % (379 of 559)68.8 % (236 of 343)68.1 % (143 of 210) 0.0 % (0 of 6)
Aromatic 4.4 % (4 of 90) 8.9 % (4 of 45) 0.0 % (0 of 45)
Methyl96.7 % (58 of 60)100.0 % (30 of 30)93.3 % (28 of 30)

1. J-domain of DnaJA1

MGHHHHHHSH MVKETTYYDV LGVKPNATQE ELKKAYRKLA LKYHPDKNPN EGEKFKQISQ AYEVLSDAKK RELYDKG

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1J-domain of DnaJA1[U-13C; U-15N]1.03 mM
2MESnatural abundance20 mM
3sodium azidenatural abundance0.02 %
4DTTnatural abundance10 mM
5calcium chloridenatural abundance5 mM
6sodium chloridenatural abundance100 mM
7DSSnatural abundance50 uM

LACS Plot; CA
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.11 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.08 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.29 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2M6Y, Strand ID: A Detail


Release date
2013-06-23
Citation
Structure and function of human DnaJ homologue subfamily a member 1 (DNAJA1) and its relationship to pancreatic cancer
Stark, J.L., Mehla, K., Chaika, N., Acton, T.B., Xiao, R., Singh, P.K., Montelione, G.T., Powers, R.
Biochemistry (2014), 53, 1360-1372, PubMed 24512202 , DOI 10.1021/bi401329a ,
Related entities 1. J-domain of human DnaJA1, : 1 : 7 : 108 entities Detail
Interaction partners 1. J-domain of human DnaJA1, : 125 interactors Detail
Experiments performed 16 experiments Detail
NMR combined restraints 5 contents Detail
Keywords protein