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BMRB: 19167

2M70

Structural determination of the Citrus sinensis Poly(A)-Binding Protein CsPABP1

Authors

Sforca, M.L., Domingues, M.N., Zeri, A.C., Benedetti, C.E.

Assembly

CsPABP1

Entity

1. CsPABP1 (polymer, Thiol state: all free), 155 monomers, 17160.90 Da Detail

MGSSHHHHHH SSGLVPRGSH MGSDLEDMKK RLKEIEEEAG ALREMQAKVE KEMGAVQDSS STSATQAEKE EVDSRSIYVG NVDYACTPEE VQQHFQSCGT VNRVTILTDK FGQPKGFAYV EFVEIDAVQN ALLLNETELH GRQLKVSAKR TNIPG

Formula weight

17160.9 Da

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.0 %, Completeness: 66.8 %, Completeness (bb): 76.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.8 % (1164 of 1742)	61.9 % (561 of 907)	71.9 % (482 of 670)	73.3 % (121 of 165)
Backbone	76.7 % (707 of 922)	75.5 % (240 of 318)	76.6 % (347 of 453)	79.5 % (120 of 151)
Sidechain	59.5 % (573 of 963)	54.5 % (321 of 589)	69.7 % (251 of 360)	7.1 % (1 of 14)
Aromatic	34.0 % (34 of 100)	32.0 % (16 of 50)	36.0 % (18 of 50)
Methyl	77.3 % (116 of 150)	74.7 % (56 of 75)	80.0 % (60 of 75)

1. CsPABP1

MGSSHHHHHH SSGLVPRGSH MGSDLEDMKK RLKEIEEEAG ALREMQAKVE KEMGAVQDSS STSATQAEKE EVDSRSIYVG NVDYACTPEE VQQHFQSCGT VNRVTILTDK FGQPKGFAYV EFVEIDAVQN ALLLNETELH GRQLKVSAKR TNIPG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	CsPABP1	[U-98% 15N]	0.6 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	20 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
6	CsPABP1	[U-99% 13C; U-99% 15N]	0.6 mM
7	sodium phosphate	natural abundance	50 mM
8	sodium chloride	natural abundance	20 mM
9	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
10	CsPABP1	[U-99% 13C; U-99% 15N]	0.6 mM
11	sodium phosphate	natural abundance	50 mM
12	sodium chloride	natural abundance	20 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M70, Strand ID: A Detail

Release date

2014-04-13