NMR solution structure of N-terminal domain of (Y81F)-EhCaBP1
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.0 % (586 of 781) | 67.8 % (276 of 407) | 83.0 % (249 of 300) | 82.4 % (61 of 74) |
Backbone | 92.2 % (365 of 396) | 90.5 % (124 of 137) | 93.3 % (180 of 193) | 92.4 % (61 of 66) |
Sidechain | 62.3 % (278 of 446) | 56.3 % (152 of 270) | 75.0 % (126 of 168) | 0.0 % (0 of 8) |
Aromatic | 1.5 % (1 of 66) | 3.0 % (1 of 33) | 0.0 % (0 of 33) | |
Methyl | 82.9 % (58 of 70) | 77.1 % (27 of 35) | 88.6 % (31 of 35) |
1. (Y81F)-EhCaBP1
MAEALFKEID VNGDGAVSYE EVKAFVSKKR AIKNEQLLQL IFKSIDADGN GEIDQNEFAK FYGSIQSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details (u-15N),(Y81F)-EhCaBP1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | (Y81F)-EhCaBP1 | [U-99% 15N] | 0.8 (±0.1) mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details (u-15N/13C), (Y81F)-EhCaBP1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | (Y81F)-EhCaBP1 | [U-99% 13C; U-99% 15N] | 0.8 (±0.1) mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details (u-15N/13C), (Y81F)-EhCaBP1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | (Y81F)-EhCaBP1 | [U-99% 13C; U-99% 15N] | 0.8 (±0.1) mM | |
8 | D2O | natural abundance | 100 % |