BMRB: 19252

RRM domain of HUMAN RBM7

Authors

Sikorsky, T., Hrossova, D., Vanacova, S., Stefl, R.

Assembly

RRM domain of HUMAN RBM7

Entity

1. RRM domain of HUMAN RBM7 (polymer, Thiol state: not present), 101 monomers, 11455.03 Da Detail

GSEFELRRQQ ALAEADRTLF VGNLETKVTE ELLFELFHQA GPVIKVKIPK DKDGKPKQFA FVNFKHEVSV PYAMNLLNGI KLYGRPIKIQ FRSGSSHAPQ D

Formula weight

11455.03 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.1 %, Completeness: 75.7 %, Completeness (bb): 72.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.7 % (935 of 1235)	80.2 % (521 of 650)	68.5 % (329 of 480)	81.0 % (85 of 105)
Backbone	72.2 % (429 of 594)	81.3 % (165 of 203)	63.2 % (187 of 296)	81.1 % (77 of 95)
Sidechain	80.0 % (588 of 735)	79.6 % (356 of 447)	80.6 % (224 of 278)	80.0 % (8 of 10)
Aromatic	55.6 % (60 of 108)	55.6 % (30 of 54)	55.6 % (30 of 54)
Methyl	96.3 % (104 of 108)	96.3 % (52 of 54)	96.3 % (52 of 54)

1. RBM7

GSEFELRRQQ ALAEADRTLF VGNLETKVTE ELLFELFHQA GPVIKVKIPK DKDGKPKQFA FVNFKHEVSV PYAMNLLNGI KLYGRPIKIQ FRSGSSHAPQ D

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	RBM7	[U-99% 13C; U-99% 15N]		0.3 mM
2	BISTRIS	natural abundance		20 mM
3	beta-mercaptoethanol	natural abundance		4 mM
4	sodium chloride	natural abundance		200 mM

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	[15N] nitromethane	nitrogen	0.0 ppm	external	direct	1.0

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	[15N] nitromethane	nitrogen	0.0 ppm	external	direct	1.0

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	[15N] nitromethane	nitrogen	0.0 ppm	external	direct	1.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2M8H, Strand ID: A Detail

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Release date

2015-01-25

Citation

RNA binding properties of the human NEXT complex
Sikorsky, T., Stefl, R., Hrossova, D., Vanacova, S.
Not known

Related entities 1. RRM domain of HUMAN RBM7, : 1 : 5 : 148 entities Detail

Interaction partners 1. RRM domain of HUMAN RBM7, : 32 interactors Detail

Experiments performed 6 experiments Detail