The structure of the W184AM185A mutant of the HIV-1 capsid protein
PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNAATETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV L
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 44.5 % (1184 of 2663) | 22.5 % (313 of 1390) | 64.3 % (663 of 1031) | 86.0 % (208 of 242) |
Backbone | 80.2 % (1083 of 1350) | 50.6 % (234 of 462) | 95.4 % (644 of 675) | 96.2 % (205 of 213) |
Sidechain | 19.7 % (300 of 1526) | 8.4 % (78 of 928) | 38.7 % (220 of 569) | 6.9 % (2 of 29) |
Aromatic | 0.0 % (0 of 144) | 0.0 % (0 of 72) | 0.0 % (0 of 68) | 0.0 % (0 of 4) |
Methyl | 16.9 % (45 of 266) | 12.0 % (16 of 133) | 21.8 % (29 of 133) |
1. entity
PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNAATETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV LSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-13C; U-15N; U-2H] | 0.5 mM | |
2 | EDTA | natural abundance | 1 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | sodium phosphate | natural abundance | 20 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | natural abundance | 5 % |