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Structure of d[CGCGAAGCATTCGCG] hairpin
Authors
Lim, K., Phan, A.
Assembly
d[CGCGAAGCATTCGCG] hairpin
Entity
1. d[CGCGAAGCATTCGCG] hairpin (polymer, Thiol state: not present), 15 monomers, 4657.958 Da Detail

CGCGAAGCAT TCGCG


Formula weight
4657.958 Da
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 91.8 %, Completeness (bb): 100.0 % Detail

Polymer type: polydeoxyribonucleotide

Total1H
All91.8 % (135 of 147)91.8 % (135 of 147)
Suger, PO4100.0 % (105 of 105)100.0 % (105 of 105)
Nucleobase71.4 % (30 of 42)71.4 % (30 of 42)
Aromatic93.3 % (28 of 30)93.3 % (28 of 30)
Methyl100.0 % (2 of 2)100.0 % (2 of 2)

1. DNA (5'-D(*CP*GP*CP*GP*AP*AP*GP*CP*AP*TP*TP*CP*GP*CP*G)-3')

CGCGAAGCAT TCGCG

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*CP*GP*CP*GP*AP*AP*GP*CP*AP*TP*TP*CP*GP*CP*G)-3')natural abundance0.5-2.0 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
4DNA (5'-D(*CP*GP*CP*GP*AP*AP*GP*CP*AP*TP*TP*CP*GP*CP*G)-3')natural abundance0.5-2.0 mM
5D2Onatural abundance100 %
Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
6DNA (5'-D(*CP*GP*CP*GP*AP*AP*GP*CP*AP*TP*TP*CP*GP*CP*G)-3')[U-2% 15N]0.5-2.0 mM
7H2Onatural abundance90 %
8D2Onatural abundance10 %
Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
9DNA (5'-D(*CP*GP*CP*GP*AP*AP*GP*CP*AP*TP*TP*CP*GP*CP*G)-3')[U-100% 2H]0.5-2.0 mM
10D2Onatural abundance100 %

Release date
2013-07-07
Citation
Structural basis of DNA quadruplex-duplex junction formation
Lim, K., Phan, A.
Angew. Chem Int Ed Engl. (2013), 52, 8566-8569, PubMed 23794476 , DOI 10.1002/anie.201302995 ,
Experiments performed 10 experiments Detail
NMR combined restraints 6 contents Detail
Keywords DNA, duplex, hairpin