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Structure of d[TTGGGTGGGCGCGAAGCATTCGCGGGGTGGGT] duplex-quadruplex hybrid
Authors
Lim, K., Phan, A.
Assembly
d[TTGGGTGGGCGCGAAGCATTCGCGGGGTGGGT] duplex-quadruplex hybrid
Entity
1. d[TTGGGTGGGCGCGAAGCATTCGCGGGGTGGGT] duplex-quadruplex hybrid (polymer, Thiol state: not present), 32 monomers, 10129.39 Da Detail

TTGGGTGGGC GCGAAGCATT CGCGGGGTGG GT


Formula weight
10129.39 Da
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 80.7 %, Completeness (bb): 78.6 % Detail

Polymer type: polydeoxyribonucleotide

Total1H
All80.7 % (246 of 305)80.7 % (246 of 305)
Suger, PO478.6 % (176 of 224)78.6 % (176 of 224)
Nucleobase86.4 % (70 of 81)86.4 % (70 of 81)
Aromatic89.1 % (57 of 64)89.1 % (57 of 64)
Methyl100.0 % (7 of 7)100.0 % (7 of 7)

1. DNA (32-MER)

TTGGGTGGGC GCGAAGCATT CGCGGGGTGG GT

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
1DNA (32-MER)natural abundance0.5-2.0 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
4DNA (32-MER)natural abundance0.5-2.0 mM
Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
5DNA (32-MER)[U-2% 15N]0.5-2.0 mM
Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7


#NameIsotope labelingTypeConcentration
6DNA (32-MER)[U-100% 2H]0.5-2.0 mM

Release date
2013-07-07
Citation
Structural basis of DNA quadruplex-duplex junction formation
Lim, K., Phan, A.
Angew. Chem Int Ed Engl. (2013), 52, 8566-8569, PubMed 23794476 , DOI 10.1002/anie.201302995 ,
Experiments performed 10 experiments Detail
NMR combined restraints 6 contents Detail
Keywords DNA, duplex, duplex-quadruplex hybrid, quadruplex