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BMRB: 19290


19290
4BS2
NMR structure of human TDP-43 tandem RRMs in complex with UG-rich RNA
Authors
Lukavsky, P.JOSEF., Daujotyte, D., Tollervey, J.R., Ule, J., Stuani, C., Buratti, E., Baralle, F.E., Damberger, F.F., Allain, F.H.T.
Assembly
NMR structure of human TDP-43 tandem RRMs in complex with UG-rich RNA
Entity
1. TAR DNA-BINDING PROTEIN 43 (polymer, Thiol state: all free), 174 monomers, 19967.48 Da Detail

GSHMASKTSD LIVLGLPWKT TEQDLKEYFS TFGEVLMVQV KKDLKTGHSK GFGFVRFTEY ETQVKVMSQR HMIDGRWCDC KLPNSKQSQD EPLRSRKVFV GRCTEDMTED ELREFFSQYG DVMDVFIPKP FRAFAFVTFA DDQIAQSLCG EDLIIKGISV HISNAEPKHN SNRQ


2. 5'-R(*GP*UP*GP*UP*GP*AP*AP*UP*GP*AP*AP*UP)-3' (polymer, Thiol state: not present), 12 monomers, 3940.324 Da Detail

GUGUGAAUGA AU


Total weight
23907.805 Da
Max. entity weight
19967.48 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
SIMULATED ANNEALING COMBINED WITH MOLECULAR DYNAMICS IN TORSION ANGLE SPACE
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.9 %, Completeness: 96.9 %, Completeness (bb): 96.6 % Detail
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Polymer type: polypeptide(L) polyribonucleotide

Total1H13C15N31P
All96.9 % (2205 of 2276)97.2 % (1152 of 1185)97.6 % (866 of 887)91.7 % (176 of 192)91.7 % (11 of 12)
Backbone96.6 % (1136 of 1176)96.7 % (411 of 425)96.8 % (553 of 571)95.8 % (161 of 168)91.7 % (11 of 12)
Sidechain97.5 % (1231 of 1263)97.5 % (741 of 760)99.2 % (475 of 479)62.5 % (15 of 24)
Aromatic95.3 % (242 of 254)96.9 % (123 of 127)100.0 % (117 of 117)20.0 % (2 of 10)
Methyl99.4 % (159 of 160)100.0 % (80 of 80)98.8 % (79 of 80)

1. TAR DNA-BINDING PROTEIN 43

GSHMASKTSD LIVLGLPWKT TEQDLKEYFS TFGEVLMVQV KKDLKTGHSK GFGFVRFTEY ETQVKVMSQR HMIDGRWCDC KLPNSKQSQD EPLRSRKVFV GRCTEDMTED ELREFFSQYG DVMDVFIPKP FRAFAFVTFA DDQIAQSLCG EDLIIKGISV HISNAEPKHN SNRQ

2. 5'-R(*GP*UP*GP*UP*GP*AP*AP*UP*GP*AP*AP*UP)-3'

GUGUGAAUGA AU

Show sample condition
Sample

Solvent system 94%water/6%D2O, Pressure 1 atm, Temperature 298.000 K, pH 6.800, Details 0.7 mmol/l


#NameIsotope labelingTypeConcentration
1TAR DNA-BINDING PROTEIN 43[U-13C; U-15N]0.7 mM
25'-R(*GP*UP*GP*UP*GP*AP*AP*UP*GP*AP*AP*UP)-3'natural abundance0.7 mM

LACS Plot; CA
Referencing offset: -0.05 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.05 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.1 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.3 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 4BS2, Strand ID: A Detail

Release date
2013-06-27
Citation
Molecular basis of UG-rich RNA recognition by the human splicing factor TDP-43
Lukavsky, P.J., Daujotyte, D., Tollervey, J.R., Ule, J., Stuani, C., Buratti, E., Baralle, F.E., Damberger, F.F., Allain, F.
Nat. Struct. Mol. Biol. (2013), 20, 1443-1449, PubMed 24240615 , DOI 10.1038/nsmb.2698 ,
Related entities 1. TAR DNA-BINDING PROTEIN 43, : 1 : 10 : 169 entities Detail
More details for 180 related entities
Related entities 2. 5'-R(*GP*UP*GP*UP*GP*AP*AP*UP*GP*AP*AP*UP)-3', : 1 entities Detail
More details for 1 related entities
Interaction partners 1. TAR DNA-BINDING PROTEIN 43, : 74 interactors Detail
More details for 74 interactors identified by 29 citations
Experiments performed 5 experiments Detail
nullKeywords alternative splicing, cystic fibrosis, hammerhead ribozyme, HAMMERHEA RIBOZYME, hnRNP, isotope-labelled RNA, ISOTOPE- LABELLED RNA, NEURODEGENERA SOLUTION NMR SPECTROSCOPY, neurodegeneration, RNA-protein complex, solution NMR spectroscopy, TDP-43, TRANSCRIPTION