Solution NMR Structure of yahO protein from Salmonella typhimurium, Northeast Structural Genomics Consortium (NESG) Target StR106
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.8 % (810 of 892) | 91.6 % (423 of 462) | 89.2 % (313 of 351) | 93.7 % (74 of 79) |
Backbone | 91.6 % (427 of 466) | 91.9 % (147 of 160) | 91.3 % (209 of 229) | 92.2 % (71 of 77) |
Sidechain | 88.8 % (443 of 499) | 90.4 % (273 of 302) | 86.2 % (168 of 195) | 100.0 % (2 of 2) |
Aromatic | 42.6 % (23 of 54) | 55.6 % (15 of 27) | 29.6 % (8 of 27) | |
Methyl | 97.6 % (82 of 84) | 97.6 % (41 of 42) | 97.6 % (41 of 42) |
1. StR106
AEIMKKTDFD KVASEYTKIG TISTTGEMSP LDAREDLIKK ADEKGADVVV LTSGQTENKI HGTADIYKKK LEHHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.5, Details 1.1 mM [U-13C;15N]-StR106, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | StR106 | [U-100% 13C; U-100% 15N] | 1.1 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | DTT | natural abundance | 10 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | sodium chloride | natural abundance | 100 mM | |
6 | ammonium acetate | natural abundance | 20 mM | |
7 | DSS | natural abundance | 50 uM | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.5, Details 1 mM [5% 13C;U-100% 15N]-StR106, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | StR106 | [5% 13C; U-100% 15N] | 1 mM | |
11 | NaN3 | natural abundance | 0.02 % | |
12 | DTT | natural abundance | 10 mM | |
13 | calcium chloride | natural abundance | 5 mM | |
14 | sodium chloride | natural abundance | 100 mM | |
15 | ammonium acetate | natural abundance | 20 mM | |
16 | DSS | natural abundance | 50 uM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 4.5, Details 0.3 mM [U-13C;15N]-StR106, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | StR106 | [U-100% 13C; U-100% 15N] | 0.3 mM | |
20 | NaN3 | natural abundance | 0.02 % | |
21 | DTT | natural abundance | 10 mM | |
22 | calcium chloride | natural abundance | 5 mM | |
23 | sodium chloride | natural abundance | 100 mM | |
24 | ammonium acetate | natural abundance | 20 mM | |
25 | DSS | natural abundance | 50 uM | |
26 | D2O | natural abundance | 100 % |