HIV-1 Vif SOCS-box and Elongin BC solution structure
MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ
VKLISSDGHE FIVKREHALT SGTIKAMLSG PGQFAENETN EVNFREIPSH VLSKVCMYFT YKVRYTNSST EIPEFPIAPE IALELLMAAN F
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 10.7 % (308 of 2881) | 8.2 % (123 of 1504) | 11.9 % (135 of 1132) | 20.4 % (50 of 245) |
Backbone | 18.8 % (269 of 1434) | 19.5 % (94 of 483) | 17.5 % (127 of 724) | 21.1 % (48 of 227) |
Sidechain | 5.4 % (91 of 1681) | 2.8 % (29 of 1021) | 9.3 % (60 of 642) | 11.1 % (2 of 18) |
Aromatic | 0.0 % (0 of 202) | 0.0 % (0 of 101) | 0.0 % (0 of 100) | 0.0 % (0 of 1) |
Methyl | 4.4 % (12 of 274) | 2.9 % (4 of 137) | 5.8 % (8 of 137) |
1. Vif SOCS
HNKVGSLQYL ALAALIKPKQ IKPPLPSVRK LTEDRW2. Elongin B
MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ3. Elongin C
VKLISSDGHE FIVKREHALT SGTIKAMLSG PGQFAENETN EVNFREIPSH VLSKVCMYFT YKVRYTNSST EIPEFPIAPE IALELLMAAN FSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details labeled SOCS in the presence of unlabeled EloBC
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Vif SOCS | [U-99% 13C; U-99% 15N] | 0.2 mM | |
2 | Elongin B | natural abundance | 0.2 mM | |
3 | Elongin C | natural abundance | 0.2 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % | |
6 | Na-phosphate buffer | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details labeled EloBC in the presence of unlabeled SOCS
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Vif SOCS | natural abundance | 0.2 mM | |
8 | Elongin B | [U-99% 13C; U-99% 15N] | 0.2 mM | |
9 | Elongin C | [U-99% 13C; U-99% 15N] | 0.2 mM | |
10 | H2O | natural abundance | 90 % | |
11 | D2O | natural abundance | 10 % | |
12 | Na-phosphate buffer | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 100% Deuterated labeled EloBC in the presence of unlabeled SOCS
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | Vif SOCS | natural abundance | 0.2 mM | |
14 | Elongin B | [U-99% 13C; U-99% 15N] | 0.2 mM | |
15 | Elongin C | [U-99% 13C; U-99% 15N] | 0.2 mM | |
16 | H2O | natural abundance | 90 % | |
17 | D2O | natural abundance | 10 % | |
18 | Na-phosphate buffer | natural abundance | 50 mM |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 14.3 % (826 of 5762) | 9.3 % (281 of 3008) | 17.6 % (398 of 2264) | 30.0 % (147 of 490) |
Backbone | 26.8 % (768 of 2868) | 24.5 % (237 of 966) | 26.7 % (386 of 1448) | 31.9 % (145 of 454) |
Sidechain | 4.7 % (158 of 3362) | 2.2 % (44 of 2042) | 8.7 % (112 of 1284) | 5.6 % (2 of 36) |
Aromatic | 0.0 % (0 of 404) | 0.0 % (0 of 202) | 0.0 % (0 of 200) | 0.0 % (0 of 2) |
Methyl | 3.8 % (21 of 548) | 2.6 % (7 of 274) | 5.1 % (14 of 274) |
1. Vif SOCS
HNKVGSLQYL ALAALIKPKQ IKPPLPSVRK LTEDRW2. Elongin B
MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ3. Elongin C
VKLISSDGHE FIVKREHALT SGTIKAMLSG PGQFAENETN EVNFREIPSH VLSKVCMYFT YKVRYTNSST EIPEFPIAPE IALELLMAAN FSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details labeled SOCS in the presence of unlabeled EloBC
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Vif SOCS | [U-99% 13C; U-99% 15N] | 0.2 mM | |
2 | Elongin B | natural abundance | 0.2 mM | |
3 | Elongin C | natural abundance | 0.2 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % | |
6 | Na-phosphate buffer | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details labeled EloBC in the presence of unlabeled SOCS
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Vif SOCS | natural abundance | 0.2 mM | |
8 | Elongin B | [U-99% 13C; U-99% 15N] | 0.2 mM | |
9 | Elongin C | [U-99% 13C; U-99% 15N] | 0.2 mM | |
10 | H2O | natural abundance | 90 % | |
11 | D2O | natural abundance | 10 % | |
12 | Na-phosphate buffer | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 100% Deuterated labeled EloBC in the presence of unlabeled SOCS
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | Vif SOCS | natural abundance | 0.2 mM | |
14 | Elongin B | [U-99% 13C; U-99% 15N] | 0.2 mM | |
15 | Elongin C | [U-99% 13C; U-99% 15N] | 0.2 mM | |
16 | H2O | natural abundance | 90 % | |
17 | D2O | natural abundance | 10 % | |
18 | Na-phosphate buffer | natural abundance | 50 mM |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 12.7 % (1101 of 8643) | 8.3 % (374 of 4512) | 15.7 % (532 of 3396) | 26.5 % (195 of 735) |
Backbone | 23.6 % (1016 of 4302) | 21.3 % (309 of 1449) | 23.7 % (514 of 2172) | 28.3 % (193 of 681) |
Sidechain | 4.2 % (211 of 5043) | 2.1 % (65 of 3063) | 7.5 % (144 of 1926) | 3.7 % (2 of 54) |
Aromatic | 0.0 % (0 of 606) | 0.0 % (0 of 303) | 0.0 % (0 of 300) | 0.0 % (0 of 3) |
Methyl | 4.1 % (34 of 822) | 3.2 % (13 of 411) | 5.1 % (21 of 411) |
1. Vif SOCS
HNKVGSLQYL ALAALIKPKQ IKPPLPSVRK LTEDRW2. Elongin B
MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ3. Elongin C
VKLISSDGHE FIVKREHALT SGTIKAMLSG PGQFAENETN EVNFREIPSH VLSKVCMYFT YKVRYTNSST EIPEFPIAPE IALELLMAAN FSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details labeled SOCS in the presence of unlabeled EloBC
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Vif SOCS | [U-99% 13C; U-99% 15N] | 0.2 mM | |
2 | Elongin B | natural abundance | 0.2 mM | |
3 | Elongin C | natural abundance | 0.2 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % | |
6 | Na-phosphate buffer | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details labeled EloBC in the presence of unlabeled SOCS
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Vif SOCS | natural abundance | 0.2 mM | |
8 | Elongin B | [U-99% 13C; U-99% 15N] | 0.2 mM | |
9 | Elongin C | [U-99% 13C; U-99% 15N] | 0.2 mM | |
10 | H2O | natural abundance | 90 % | |
11 | D2O | natural abundance | 10 % | |
12 | Na-phosphate buffer | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 100% Deuterated labeled EloBC in the presence of unlabeled SOCS
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | Vif SOCS | natural abundance | 0.2 mM | |
14 | Elongin B | [U-99% 13C; U-99% 15N] | 0.2 mM | |
15 | Elongin C | [U-99% 13C; U-99% 15N] | 0.2 mM | |
16 | H2O | natural abundance | 90 % | |
17 | D2O | natural abundance | 10 % | |
18 | Na-phosphate buffer | natural abundance | 50 mM |