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BMRB: 19380

2MAX

NMR structure of the RNA polymerase alpha subunit C-terminal domain from Helicobacter pylori

Authors

Borin, B.N., Krezel, A.M.

Assembly

RNAP alpha subunit CTD

Entity

1. RNAP alpha subunit CTD (polymer, Thiol state: all free), 126 monomers, 14134.70 Da Detail

MRGSHHHHHH GSLGVFGERP IANTEYSGDY AQRDDAKDLS AKIESMNLSA RCFNCLDKIG IKYVGELVLM SEEELKGVKN MGKKSYDEIA EKLNDLGYPV GTELSPEQRE SLKKRLEKLE DKGGND

Formula weight

14134.7 Da

Source organism

Helicobacter pylori

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 78.1 %, Completeness (bb): 77.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.1 % (1131 of 1448)	84.0 % (641 of 763)	67.5 % (374 of 554)	88.5 % (116 of 131)
Backbone	77.3 % (580 of 750)	87.8 % (230 of 262)	65.5 % (239 of 365)	90.2 % (111 of 123)
Sidechain	80.5 % (653 of 811)	82.0 % (411 of 501)	78.5 % (237 of 302)	62.5 % (5 of 8)
Aromatic	26.2 % (22 of 84)	52.4 % (22 of 42)	0.0 % (0 of 42)
Methyl	96.3 % (104 of 108)	96.3 % (52 of 54)	96.3 % (52 of 54)

1. RNAP alpha subunit CTD

MRGSHHHHHH GSLGVFGERP IANTEYSGDY AQRDDAKDLS AKIESMNLSA RCFNCLDKIG IKYVGELVLM SEEELKGVKN MGKKSYDEIA EKLNDLGYPV GTELSPEQRE SLKKRLEKLE DKGGND

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.3, Details 15N labeled

#	Name	Isotope labeling	Concentration
1	RNAP alpha subunit CTD	[U-15N]	1 mM
2	potassium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	225 mM
4	TCEP	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.3, Details 15N,13C labeled

#	Name	Isotope labeling	Concentration
7	RNAP alpha subunit CTD	[U-13C; U-15N]	1 mM
8	potassium phosphate	natural abundance	25 mM
9	potassium chloride	natural abundance	225 mM
10	TCEP	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2MAX, Strand ID: A Detail

Release date

2014-02-12

Citation

Helicobacter pylori RNA polymerase α-subunit C-terminal domain shows features unique to ɛ-proteobacteria and binds NikR/DNA complexes
Borin, B.N., Tang, W., Krezel, A.M.
Protein Sci. (2014), 23, 454-463, PubMed 24442709 , DOI 10.1002/pro.2427 , Abstract

Related entities 1. RNAP alpha subunit CTD, : 1 : 3 : 11 entities Detail

Interaction partners 1. RNAP alpha subunit CTD, : 2 interactors Detail

More details for 2 interactors identified by 1 citations