Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'
MVEADRPGKL FIGGLNTETN EKALEAVFGK YGRIVEVLLM KDRETNKSRG FAFVTFESPA DAKDAARDMN GKSLDGKAIK VEQATKPSFE SGRRG
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.0 % (979 of 1180) | 93.6 % (581 of 621) | 65.7 % (303 of 461) | 96.9 % (95 of 98) |
Backbone | 77.9 % (491 of 630) | 94.8 % (221 of 233) | 59.0 % (180 of 305) | 97.8 % (90 of 92) |
Sidechain | 90.1 % (572 of 635) | 92.8 % (360 of 388) | 85.9 % (207 of 241) | 83.3 % (5 of 6) |
Aromatic | 68.1 % (64 of 94) | 93.6 % (44 of 47) | 43.5 % (20 of 46) | 0.0 % (0 of 1) |
Methyl | 100.0 % (90 of 90) | 100.0 % (45 of 45) | 100.0 % (45 of 45) |
1. hnRNP G RRM
MVEADRPGKL FIGGLNTETN EKALEAVFGK YGRIVEVLLM KDRETNKSRG FAFVTFESPA DAKDAARDMN GKSLDGKAIK VEQATKPSFE SGRRG2. RNA AUCAAA
AUCAAASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hnRNP_G_RRM | [U-100% 15N] | 1 mM | |
2 | RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') | natural abundance | 1 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % | |
5 | NaCl | natural abundance | 50 mM | |
6 | NaH2PO4 | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | hnRNP_G_RRM | [U-100% 15N] | 1 mM | |
8 | RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') | natural abundance | 1 mM | |
9 | D2O | natural abundance | 100 % | |
10 | NaCl | natural abundance | 50 mM | |
11 | NaH2PO4 | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | hnRNP_G_RRM | [U-100% 13C; U-100% 15N] | 1 mM | |
13 | RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') | natural abundance | 1 mM | |
14 | H2O | natural abundance | 90 % | |
15 | D2O | natural abundance | 10 % | |
16 | NaCl | natural abundance | 50 mM | |
17 | NaH2PO4 | natural abundance | 20 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | hnRNP_G_RRM | [U-100% 13C; U-100% 15N] | 1 mM | |
19 | RNA (5'-R(*AP*UP*CP*AP*AP*A)-3') | natural abundance | 1 mM | |
20 | D2O | natural abundance | 100 % | |
21 | NaCl | natural abundance | 50 mM | |
22 | NaH2PO4 | natural abundance | 20 mM |