BMRBj - BMREntryMaster

Found 1 Document (0.96 seconds)

BMRB: 19382

2MB0

Solution structure of hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'

Authors

Moursy, A., Allain, F.H.T., Clery, A.

Assembly

hnRNP G RRM in complex with the RNA 5'-AUCAAA-3'

Entity

1. hnRNP G RRM (polymer, Thiol state: not present), 95 monomers, 10396.68 Da Detail

MVEADRPGKL FIGGLNTETN EKALEAVFGK YGRIVEVLLM KDRETNKSRG FAFVTFESPA DAKDAARDMN GKSLDGKAIK VEQATKPSFE SGRRG

2. RNA AUCAAA (polymer, Thiol state: not present), 6 monomers, 1946.186 Da Detail

AUCAAA

Total weight

12342.865 Da

Max. entity weight

10396.68 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 83.0 %, Completeness (bb): 77.9 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	83.0 % (979 of 1180)	93.6 % (581 of 621)	65.7 % (303 of 461)	96.9 % (95 of 98)
Backbone	77.9 % (491 of 630)	94.8 % (221 of 233)	59.0 % (180 of 305)	97.8 % (90 of 92)
Sidechain	90.1 % (572 of 635)	92.8 % (360 of 388)	85.9 % (207 of 241)	83.3 % (5 of 6)
Aromatic	68.1 % (64 of 94)	93.6 % (44 of 47)	43.5 % (20 of 46)	0.0 % (0 of 1)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. hnRNP G RRM

MVEADRPGKL FIGGLNTETN EKALEAVFGK YGRIVEVLLM KDRETNKSRG FAFVTFESPA DAKDAARDMN GKSLDGKAIK VEQATKPSFE SGRRG

2. RNA AUCAAA

AUCAAA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	hnRNP_G_RRM	[U-100% 15N]	1 mM
2	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaCl	natural abundance	50 mM
6	NaH2PO4	natural abundance	20 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	hnRNP_G_RRM	[U-100% 15N]	1 mM
8	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
9	D2O	natural abundance	100 %
10	NaCl	natural abundance	50 mM
11	NaH2PO4	natural abundance	20 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
18	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
19	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
20	D2O	natural abundance	100 %
21	NaCl	natural abundance	50 mM
22	NaH2PO4	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	direct	1.0

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MB0, Strand ID: B Detail

Release date

2014-05-05

Citation

Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation
Moursy, A., Allain, F., Clery, A.
Nucleic Acids Res. (2014), 42, 6659-6672, PubMed 24692659 , DOI 10.1093/nar/gku244 , Abstract

Related entities 1. hnRNP G RRM, : 1 : 7 : 3 : 1 : 299 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	hnRNP_G_RRM	[U-100% 15N]	1 mM
2	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaCl	natural abundance	50 mM
6	NaH2PO4	natural abundance	20 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

4 2D 1H-1H TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	hnRNP_G_RRM	[U-100% 15N]	1 mM
8	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
9	D2O	natural abundance	100 %
10	NaCl	natural abundance	50 mM
11	NaH2PO4	natural abundance	20 mM

5 2D 1H-1H NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	hnRNP_G_RRM	[U-100% 15N]	1 mM
8	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
9	D2O	natural abundance	100 %
10	NaCl	natural abundance	50 mM
11	NaH2PO4	natural abundance	20 mM

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

7 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

8 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

9 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

10 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

11 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
12	hnRNP_G_RRM	[U-100% 13C; U-100% 15N]	1 mM
13	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %
16	NaCl	natural abundance	50 mM
17	NaH2PO4	natural abundance	20 mM

12 3D 1H-15N NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	hnRNP_G_RRM	[U-100% 15N]	1 mM
2	RNA (5'-R(APUPCPAPAPA)-3')	natural abundance	1 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %
5	NaCl	natural abundance	50 mM
6	NaH2PO4	natural abundance	20 mM

nullKeywords hnRNP G, NMR, RRM, SMA, SMN, Splicing

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Found 1 Document (0.96 seconds)

BMRB: 19382

Sample #1

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. hnRNP G RRM, : 1 : 7 : 3 : 1 : 299 entities Detail

Experiments performed 12 experiments Detail

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