Solution NMR Structure of DE NOVO DESIGNED Top7 Fold Protein Top7m13, Northeast Structural Genomics Consortium (NESG) Target OR33
MSGKKVEVQV KITCNGKTYE RTYQLYAVRD EELKEKLKKV LNERMDPIKK LGCKRVRISI RVKHSDAAEE KKEAKKFAAI LNKVFAELGY NDSNVTWDGD TVTVEGQLEG VDLEHHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.8 % (1306 of 1438) | 91.7 % (692 of 755) | 90.5 % (502 of 555) | 87.5 % (112 of 128) |
Backbone | 89.1 % (640 of 718) | 90.2 % (222 of 246) | 89.2 % (315 of 353) | 86.6 % (103 of 119) |
Sidechain | 92.4 % (770 of 833) | 92.3 % (470 of 509) | 92.4 % (291 of 315) | 100.0 % (9 of 9) |
Aromatic | 71.7 % (66 of 92) | 71.7 % (33 of 46) | 71.1 % (32 of 45) | 100.0 % (1 of 1) |
Methyl | 99.2 % (129 of 130) | 98.5 % (64 of 65) | 100.0 % (65 of 65) |
1. OR33
MSGKKVEVQV KITCNGKTYE RTYQLYAVRD EELKEKLKKV LNERMDPIKK LGCKRVRISI RVKHSDAAEE KKEAKKFAAI LNKVFAELGY NDSNVTWDGD TVTVEGQLEG VDLEHHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.69 mM OR33.017, 90% H2O/10% D2O, Low Salt NO DTT - 0.02 % NaN3 100 mM NaCl 10 mM Tris-HCl pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | OR33 | [U-100% 13C; U-100% 15N] | 0.69 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | NaCl | natural abundance | 100 mM | |
4 | Tris-HCl | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.69 mM OR33.007, 90% H2O/10% D2O, Low Salt NO DTT - 0.02 % NaN3 100 mM NaCl 10 mM Tris-HCl pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | OR33 | [U-100% 13C; U-100% 15N] | 0.69 mM | |
6 | NaN3 | natural abundance | 0.02 % | |
7 | NaCl | natural abundance | 100 mM | |
8 | Tris-HCl | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.69 mM OR33.007, 90% H2O/10% D2O, NO DTT - 0.02 % NaN3 pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | OR33 | [U-100% 13C; U-100% 15N] | 0.69 mM | |
10 | NaN3 | natural abundance | 0.02 % |