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BMRB: 19424

3QR5

Backbone chemical shift assignments of delta exon 3 mouse RyR2 domain A

Authors

Amador, F., Stathopulos, P., Seabrook, G., Ikura, M.

Assembly

RyR2A delta exon 3

Entity

1. RyR2A delta exon 3 (polymer, Thiol state: all free), 177 monomers, 19752.05 Da Detail

GSGSEIQFLR TDDEVVLQCT ATIHKEQQKL CLAAEGFGNR LCFLESTSNS KQVDVEKWKF MMKTAQGGGH RTLLYGHAIL LRHSYSGMYL CCLSTSRSST DKLAFDVGLQ EDTTGEACWW TIHPASKQRS EGEKVRVGDD LILVSVSSER YLHLSYGNSS WHVDAAFQQT LWSVAPI

Formula weight

19752.05 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.2 %, Completeness: 32.9 %, Completeness (bb): 56.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	32.9 % (665 of 2022)	19.7 % (205 of 1040)	39.5 % (312 of 789)	76.7 % (148 of 193)
Backbone	56.3 % (596 of 1058)	43.7 % (160 of 366)	55.7 % (288 of 517)	84.6 % (148 of 175)
Sidechain	18.3 % (206 of 1127)	7.0 % (47 of 674)	36.6 % (159 of 435)	0.0 % (0 of 18)
Aromatic	6.4 % (12 of 188)	6.4 % (6 of 94)	6.7 % (6 of 89)	0.0 % (0 of 5)
Methyl	17.4 % (33 of 190)	11.6 % (11 of 95)	23.2 % (22 of 95)

1. RyR2A

GSGSEIQFLR TDDEVVLQCT ATIHKEQQKL CLAAEGFGNR LCFLESTSNS KQVDVEKWKF MMKTAQGGGH RTLLYGHAIL LRHSYSGMYL CCLSTSRSST DKLAFDVGLQ EDTTGEACWW TIHPASKQRS EGEKVRVGDD LILVSVSSER YLHLSYGNSS WHVDAAFQQT LWSVAPI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details delta exon 3 RyR2A 10-217

#	Name	Isotope labeling	Type	Concentration
1	RyR2A	[U-13C; U-15N; U-2H]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		300 mM
4	DTT	natural abundance		5 mM
5	TCEP	natural abundance		2 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: 0.43 ppm, Outliers: 4 Detail

LACS Plot; CB

Referencing offset: 0.43 ppm, Outliers: 4 Detail

Release date

2014-02-11

Citation

Type 2 ryanodine receptor domain A contains a unique and dynamic α-helix that transitions to a β-strand in a mutant linked with a heritable cardiomyopathy
Amador, F.J., Kimlicka, L., Stathopulos, P.B., Gasmi-Seabrook, G.M.C., Maclennan, D.H., Van Petegem, F., Ikura, M.
J. Mol. Biol. (2013), 425, 4034-4046, PubMed 23978697 , DOI 10.1016/j.jmb.2013.08.015 , Abstract

Related entities 1. RyR2A delta exon 3, : 1 : 112 entities Detail

Interaction partners 1. RyR2A delta exon 3, : 20 interactors Detail

More details for 20 interactors identified by 8 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details delta exon 3 RyR2A 10-217

#	Name	Isotope labeling	Type	Concentration
1	RyR2A	[U-13C; U-15N; U-2H]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		300 mM
4	DTT	natural abundance		5 mM
5	TCEP	natural abundance		2 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details delta exon 3 RyR2A 10-217

#	Name	Isotope labeling	Type	Concentration
1	RyR2A	[U-13C; U-15N; U-2H]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		300 mM
4	DTT	natural abundance		5 mM
5	TCEP	natural abundance		2 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

3 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details delta exon 3 RyR2A 10-217

#	Name	Isotope labeling	Type	Concentration
1	RyR2A	[U-13C; U-15N; U-2H]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		300 mM
4	DTT	natural abundance		5 mM
5	TCEP	natural abundance		2 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

4 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details delta exon 3 RyR2A 10-217

#	Name	Isotope labeling	Type	Concentration
1	RyR2A	[U-13C; U-15N; U-2H]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		300 mM
4	DTT	natural abundance		5 mM
5	TCEP	natural abundance		2 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

5 3D HN(COCA)CB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details delta exon 3 RyR2A 10-217

#	Name	Isotope labeling	Type	Concentration
1	RyR2A	[U-13C; U-15N; U-2H]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		300 mM
4	DTT	natural abundance		5 mM
5	TCEP	natural abundance		2 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

6 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details delta exon 3 RyR2A 10-217

#	Name	Isotope labeling	Type	Concentration
1	RyR2A	[U-13C; U-15N; U-2H]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		300 mM
4	DTT	natural abundance		5 mM
5	TCEP	natural abundance		2 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

7 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details delta exon 3 RyR2A 10-217

#	Name	Isotope labeling	Type	Concentration
1	RyR2A	[U-13C; U-15N; U-2H]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		300 mM
4	DTT	natural abundance		5 mM
5	TCEP	natural abundance		2 mM
6	H2O	natural abundance		90 %
7	D2O	natural abundance		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr19424_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr19424_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	566		82.5 (chain: 1, length: 177)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 177, Sequence coverage: 82.5%
- Total number of assignments
  - ¹H chemical shifts: 145
  - ¹⁵N chemical shifts: 145
  - ¹³C chemical shifts: 276
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords beta-trefoil rescue, CPVT, delta exon 3, domain A, ryanodine receptor 2