BMRB: 19429

A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement

Authors

Liu, B., Shadrin, A., Sheppard, C., Xu, Y., Severinov, K., Matthews, S., Wigneshweraraj, S.

Assembly

bacteriophage transcription regulator complex with p7 peptide

Entity

1. bacteriophage transcription regulator complex with p7 peptide, entity 1 (polymer, Thiol state: not present), 67 monomers, 7451.957 Da Detail

NEFTQISGYV NAFGSQRGSV LTVKVENDEG WTLVEEDFDR ADYGSDPEFV AEVSSYLKRN GGIKDLT

2. bacteriophage transcription regulator complex with p7 peptide, entity 2 (polymer, Thiol state: not present), 8 monomers, 976.0840 Da Detail

DLLNLFNQ

Total weight

8428.041 Da

Max. entity weight

7451.957 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 85.8 %, Completeness (bb): 90.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.8 % (735 of 857)	86.4 % (381 of 441)	86.1 % (286 of 332)	81.0 % (68 of 84)
Backbone	90.8 % (407 of 448)	95.5 % (149 of 156)	87.6 % (191 of 218)	90.5 % (67 of 74)
Sidechain	81.3 % (388 of 477)	81.4 % (232 of 285)	85.2 % (155 of 182)	10.0 % (1 of 10)
Aromatic	80.2 % (69 of 86)	88.4 % (38 of 43)	73.8 % (31 of 42)	0.0 % (0 of 1)
Methyl	93.6 % (73 of 78)	94.9 % (37 of 39)	92.3 % (36 of 39)

1. entity 1

NEFTQISGYV NAFGSQRGSV LTVKVENDEG WTLVEEDFDR ADYGSDPEFV AEVSSYLKRN GGIKDLT

2. entity 2

DLLNLFNQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 8.7

#	Name	Isotope labeling	Type	Concentration
1	sodium chloride	natural abundance		5 mM
2	potassium phosphate	natural abundance		5 mM
3	entity_1	natural abundance		5 mM
4	entity_2	natural abundance		5 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 8.7

#	Name	Isotope labeling	Type	Concentration
7	sodium chloride	natural abundance		5 mM
8	potassium phosphate	natural abundance		5 mM
9	entity_1	natural abundance		5 mM
10	entity_2	natural abundance		5 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %

Hide sample codition

Protein Blocks Logo

Calculated from 1 models in PDB: 2MC6, Strand ID: A, B Detail

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Release date

2014-02-11

Citation

A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement
Liu, B., Shadrin, A., Sheppard, C., Mekler, V., Xu, Y., Severinov, K., Matthews, S., Wigneshweraraj, S.
Nucleic Acids Res. (2014), 42, 4294-4305, PubMed 24482445 , DOI 10.1093/nar/gku080 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. bacteriophage transcription regulator complex with p7 peptide, entity 1, : 2 entities Detail

Experiments performed 11 experiments Detail