Membrane induced structure of the mammalian tachykinin neuropeptide gamma
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 73.0 % (89 of 122) | 73.0 % (89 of 122) |
Backbone | 91.1 % (41 of 45) | 91.1 % (41 of 45) |
Sidechain | 62.3 % (48 of 77) | 62.3 % (48 of 77) |
Aromatic | 27.3 % (3 of 11) | 27.3 % (3 of 11) |
Methyl | 75.0 % (6 of 8) | 75.0 % (6 of 8) |
1. NPgamma
DAGHGQISHK RHKTDSFVGL MSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 2.4, Details The NMR samples were prepared by dissolving 4 mg of NPg in approximately 0.5ml of water (90% H2O, 10% D2O, pH 2.4). 50 mg of perdeuterated DPC was added yielding in solution a lipid concentration of 250 mM.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | natural abundance | 4 mg | |
2 | DPC | [U-100% 2H] | 250 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % |