Solid-state NMR structure of piscidin 3 in aligned 3:1 phosphatidylcholine/phosphoglycerol lipid bilayers
Polymer type: polypeptide(L)
Total | 15N | |
---|---|---|
All | 95.5 % (21 of 22) | 95.5 % (21 of 22) |
Backbone | 95.5 % (21 of 22) | 95.5 % (21 of 22) |
1. Piscidin 3
FIHHIFRGIV HAGRSIGRFL TGXSolvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Piscidin 3 | [15N]-F1S15 | 15.0 ~ 20.0 mM | |
2 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
3 | H2O | natural abundance | 100 % | |
4 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Piscidin 3 | [15N]-I2I5G8 | 15.0 ~ 20.0 mM | |
6 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
7 | H2O | natural abundance | 100 % | |
8 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | Piscidin 3 | [U-15N]-H3 | 15.0 ~ 20.0 mM | |
10 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
11 | H2O | natural abundance | 100 % | |
12 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | Piscidin 3 | [U-15N]-H4 | 15.0 ~ 20.0 mM | |
14 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
15 | H2O | natural abundance | 100 % | |
16 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | Piscidin 3 | [15N]-F6I9A12 | 15.0 ~ 20.0 mM | |
18 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
19 | H2O | natural abundance | 100 % | |
20 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | Piscidin 3 | [U-15N]-R7 | 15.0 ~ 20.0 mM | |
22 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
23 | H2O | natural abundance | 100 % | |
24 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | Piscidin 3 | [15N]-V10G13I16 | 15.0 ~ 20.0 mM | |
26 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
27 | H2O | natural abundance | 100 % | |
28 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
29 | Piscidin 3 | [U-15N]-H11 | 15.0 ~ 20.0 mM | |
30 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
31 | H2O | natural abundance | 100 % | |
32 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
33 | Piscidin 3 | [U-15N]-R14 | 15.0 ~ 20.0 mM | |
34 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
35 | H2O | natural abundance | 100 % | |
36 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
37 | Piscidin 3 | [15N]-G17F19L20 | 15.0 ~ 20.0 mM | |
38 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
39 | H2O | natural abundance | 100 % | |
40 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
41 | Piscidin 3 | [U-15N]-R18 | 15.0 ~ 20.0 mM | |
42 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
43 | H2O | natural abundance | 100 % | |
44 | phosphate buffer | natural abundance | 40 mM |
Solvent system 40 mM phosphate buffer with 100% H2O, Pressure 1 atm, Temperature 313 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
45 | Piscidin 3 | [15N]-T21G22 | 15.0 ~ 20.0 mM | |
46 | 3:1 (molar) 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine/1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol | natural abundance | 300.0 ~ 400.0 mM | |
47 | H2O | natural abundance | 100 % | |
48 | phosphate buffer | natural abundance | 40 mM |