BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	66.9 % (521 of 779)	70.6 % (290 of 411)	59.2 % (180 of 304)	79.7 % (51 of 64)
Backbone	74.6 % (288 of 386)	80.5 % (107 of 133)	68.8 % (132 of 192)	80.3 % (49 of 61)
Sidechain	57.6 % (261 of 453)	65.1 % (181 of 278)	45.3 % (78 of 172)	66.7 % (2 of 3)
Aromatic	42.1 % (16 of 38)	42.1 % (8 of 19)	38.9 % (7 of 18)	100.0 % (1 of 1)
Methyl	80.6 % (58 of 72)	83.3 % (30 of 36)	77.8 % (28 of 36)

1. HicA

DRTGSELMNS SKLIRMLEED GWRLVRVTGS AHHFKHPKKP GLVTVPHPKK DLPIGTVKSI QKSAGL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Concentration
3	HicA	[U-98% 13C; U-98% 15N]	1.0 mM
4	NaCl	natural abundance	150.0 mM
5	sodium phosphate	natural abundance	20.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -1.28 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -1.28 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 1.47 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 4C26, Strand ID: A Detail

Release date

2014-02-11

Citation

The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation
Butt, A., Higman, V.A., Williams, C., Crump, M.P., Hemsley, C.M., Harmer, N., Titball, R.W.
Biochem. J. (2014), 459, 333-344, PubMed 24502667 , DOI 10.1042/BJ20140073 , Abstract

Related entities 1. HicA, : 1 : 1 : 2 entities Detail

Experiments performed 12 experiments Detail

1 3D 1H-15N NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

2 3D 1H-15N TOCSY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

3 HNHA (H{[N]+[HA]})

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

4 3D HNCA

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

5 2D 1H-15N HSQC/HMQC

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
1	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

6 2D 1H-13C HSQC/HMQC

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

7 3D 1H-13C NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

8 3D HNCO

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

9 3D HCCH-TOCSY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

10 2D 1H-1H NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.400

#	Name	Isotope labeling	Concentration
3	HicA	[U-98% 13C; U-98% 15N]	1.0 mM
4	NaCl	natural abundance	150.0 mM
5	sodium phosphate	natural abundance	20.0 mM

11 3D 1H-13C NOESY

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400

#	Name	Isotope labeling	Type	Concentration
2	HicA	[U-98% 13C; U-98% 15N]		1.0 mM

12 HNHA

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 293.000 K, pH 7.400, Details HicA 1.0mM, NaCl 150mM, sodium phosphate 20mM

#	Name	Isotope labeling	Concentration
6	HicA	[U-98% 13C; U-98% 15N]	1.0 mM
7	NaCl	natural abundance	150.0 mM
8	sodium phosphate	natural abundance	20.0 mM

nullKeywords HicA, toxin

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BMRB: 19464

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. HicA, : 1 : 1 : 2 entities Detail

Experiments performed 12 experiments Detail

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